PXD070415

PXD070415 is an original dataset announced via ProteomeXchange.

Title	Coronaviruses hijack Golgi apparatus derived COPI vesicles as a membrane source for viral DMV biogenesis
Description	Using APEX2-mediated proximity labeling of SARS-CoV-2 nsp3, we identified the GBF1 as a key interacting protein within DMVs. Functional disruption of GBF1 or COPI not only suppressed viral RNA synthesis but also significantly reduced the size and abundance of DMVs and inhibits Golgi-DMVs colocalization. Imaging and biochemical assays further confirmed coronavirus infection induced redistribution of Golgi membranes to DMV formation sites. Our study demonstrates that coronaviruses exploit the GBF1-ARF1-COPI pathway to hijack COPI vesicles for the generation of DMVs, providing a mechanistic foundation for targeting this host secretory pathway as a therapeutic strategy against coronavirus infections.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-15_04:46:36.510.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhaohuan Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-06 10:22:46	ID requested
⏵ 1	2026-06-15 04:46:37	announced

Dataset with its publication pending

submitter keyword: coronavirus replication

Deyin Guo
contact affiliation	Guangzhou National Laboratory, Guangzhou International Bio-Island, Guangzhou 510000, China
contact email	guo_deyin@gzlab.ac.cn
lab head
zhaohuan Wang
contact affiliation	Guangzhou National Laboratory
contact email	wang_chaohuan@gzlab.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD070415

PRIDE project URI

• PRIDE
  1. PXD070415
     1. Label: PRIDE project
     2. Name: Coronaviruses hijack Golgi apparatus derived COPI vesicles as a membrane source for viral DMV biogenesis