PXD070387

PXD070387 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis reveals the inflammatory regulation and immune activation of mild magnetic hyperthermia in bladder cancer
Description	Inflammatory responses and immune regulation in bladder cancer treatment are critical to therapeutic efficacy, yet their specific mechanisms and protein-level changes remain unclear. Previous studies have shown that an immunosuppressive tumor microenvironment and excessive neutrophil infiltration lead to poor therapeutic safety and efficacy. In this study, a mild magnetic hyperthermia (mMHT) protocol was employed to perform low-temperature tumor magnetic hyperthermia, successfully achieving tumor inhibition and inflammation modulation in a bladder cancer-bearing mouse model. Through proteomic analysis of tumor tissues from treated mice, the mechanisms of mMHT against bladder cancer were elucidated, particularly its roles in immune activation and inflammation suppression. mMHT induces significant global protein expression reprogramming, and principal component analysis (PCA) revealed clear distinctions among the five groups, indicating distinct proteomic profiles. Differential protein analysis showed that, compared with the control group (MB49a), the mMHT group (MB49e) exhibited 2,391 upregulated proteins and 3,133 downregulated proteins, with the most pronounced differences observed relative to the three single-treatment groups. Venn analysis identified 3,259 unique differential proteins in the mMHT group, highlighting the synergistic molecular response network triggered by the combination of magnetic hyperthermia and NO therapy.
HostingRepository	iProX
AnnounceDate	2025-11-05
AnnouncementXML	Submission_2025-11-05_17:39:38.818.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiduo Sun
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-05 17:39:18	ID requested
⏵ 1	2025-11-05 17:39:39	announced

Dataset with its publication pending

submitter keyword: Mouse bladder tumor, timsTOF HT, LC-MS/MS

Jiawei Liu
contact affiliation	Institute of Geology and Geophysics, Chinese Academy of Sciences
contact email	liujiawei@mail.iggcas.ac.cn
lab head
Zhiduo Sun
contact affiliation	Institute of Geology and Geophysics, Chinese Academy of Sciences
contact email	szd2025@126.com
dataset submitter

iProX dataset URI