PXD070378

PXD070378 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling on phosphatidic acid-enriched membranes generated via optogenetic membrane editing
Description	Cellular lipid metabolism is subject to strong homeostatic regulation, but players involved in and mechanisms underlying these pathways remain mostly uncharacterized. Here, we develop and exploit a “Feeding-Fishing” approach coupling membrane editing using optogenetic lipid-modifying enzymes (feeding) with organelle membrane proteomics via proximity labeling (fishing) to elucidate molecular players and pathways involved in homeostasis of phosphatidic acid (PA), a multifunctional lipid central to glycerolipid metabolism. By performing proximity biotinylation using a membrane tethered TurboID alongside membrane editing to selectively deliver PA to the same membrane, we identified numerous PA-metabolizing enzymes and lipid transfer proteins enriched in and depleted from PA-fed membranes. Subsequent mechanistic analysis revealed that PA homeostasis in the cytosolic leaflets of the plasma membrane and lysosomes is mediated by both local PA metabolism and the action of members of the lipid transfer protein families that carry out interorganelle lipid transport prior to additional metabolic steps. More broadly, the interfacing of membrane editing with organelle membrane proteomics using proximity labeling represents a strategy for revealing mechanisms governing lipid homeostasis.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:09:40.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qin Fu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-05 13:57:28	ID requested
⏵ 1	2026-02-22 17:09:41	announced

10.1038/s41589-025-02104-x;

Tei R, Li XL, Luan L, Baskin JM, Membrane editing with proximity labeling reveals regulators of lipid homeostasis. Nat Chem Biol, ():(2026) [pubmed]

submitter keyword: Feeding-Fishing, membrane-TurboID, optoPLD, membrane editing, phospholipase D,Phosphatidic acid

Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
lab head
Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD070378

PRIDE project URI

• PRIDE
  1. PXD070378
     1. Label: PRIDE project
     2. Name: Proximity labeling on phosphatidic acid-enriched membranes generated via optogenetic membrane editing