PXD070375 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Title: A SWI/SNF-specific Ig-like domain, SWIFT, is a transcription factor binding platform |
| Description | Mammalian SWI/SNF (BAF) chromatin remodeling complexes modulate DNA accessibility and gene expression, however, the mechanisms by which they are targeted on chromatin remain incompletely understood. Here, we define SWIFT (SWI/SNF Ig-Fold for Transcription Factor Interactions), found on the SMARCD family of subunits within the core module as an evolutionarily conserved, broad transcription factor (TF) binding platform. SWIFT is necessary and sufficient for direct interaction with the transactivation domain of a lineage-specific TF, PU.1, in vitro and in cells, with a single amino acid mutation in SWIFT able to disrupt PU.1-mSWI/SNF binding, inhibit site-specific complex targeting and activity, and attenuate oncogenic gene expression and proliferation of PU.1-dependent cancer cells. Dominant expression of SWIFT in isolation across cell types sequesters mSWI/SNF-interacting TFs and poisons TF-addicted cancer cells. Finally, TFs interact with SWIFT in a SMARCD paralog-specific manner, informing approaches for modulation of cell type- and disease-specific transcription. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-31 |
| AnnouncementXML | Submission_2025-12-31_08:51:59.691.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD070375 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Alexander Ying |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-05 12:18:04 | ID requested | |
| ⏵ 1 | 2025-12-31 08:52:00 | announced | |
Publication List
| 10.6019/PXD070375; |
| 10.1101/2025.08.01.667725; |
| Jain SU, Williamson KE, Ying AW, Jiang RJ, Turner AM, So K, Allison MP, Sankar A, S, รก, me Guerra DD, Mashtalir N, Rohrs HW, Lichti CF, Gygi SP, Paulo JA, Gross ML, Kadoch C, A SWI/SNF-specific Ig-like domain, SWIFT, is a transcription factor binding platform. bioRxiv, ():(2025) [pubmed] |
Keyword List
Contact List
| Cigall Kadoch |
|---|
| contact affiliation | Dana-Farber Cancer Institute Harvard Medical School Broad Institute Howard Hughes Medical Institute |
| contact email | cigall_kadoch@dfci.harvard.edu |
| lab head | |
| Alexander Ying |
|---|
| contact affiliation | Broad Institute
Dana-Farber Cancer Institute
Harvard Medical School |
| contact email | yingalex@broadinstitute.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070375 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070375
- Label: PRIDE project
- Name: Title: A SWI/SNF-specific Ig-like domain, SWIFT, is a transcription factor binding platform
