PXD070373

PXD070373 is an original dataset announced via ProteomeXchange.

Title	Neurospora crassa Δccg8 compromises cell surface integrity and antifungal tolerance: Insights from in vitro and Galleria mellonella studies
Description	When studying filamentous fungi in terms of adaptation to environmental conditions, a reference organism is generally missing. This is mainly obstructing in the research of pathogens (well defined model organisms are non-pathogenic). Here, we investigated the mechanism underlying the ability of N. crassa to respond to various environmental stimuli, including the presence of azole and echinocandin antifungals. Our molecular analysis revealed that the Δccg8 deletion impacts the fungus on a broad physiological level, including dysregulation of ribosome biogenesis and fatty acid β-oxidation (as demonstrated by proteomic profiling), and supporting changes in glycosylated proteins and fatty acid and ergosterol composition. To bridge the gap between in vitro and in vivo conditions, we successfully applied Galleria mellonella larval model. Despite being non-pathogenic, when injected with conidia of tested filamentous fungi, the immune system cleared conidia of Δccg8 mutant strain more effectively than wild-type strain, mirroring in vitro observations and demonstrating a reproducible immune reaction. This work not only provides molecular insights into the fungal stress adaptation but also establishes N. crassa as a viable non-pathogenic organism for in vivo analysis, substantially broadening its application, reaching into dominion of research of pathogenic filamentous fungi.
HostingRepository	PRIDE
AnnounceDate	2026-03-20
AnnouncementXML	Submission_2026-03-20_00:48:41.349.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD070373
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peter Barath
SpeciesList	scientific name: Neurospora crassa; NCBI TaxID: NEWT:5141;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-05 11:53:02	ID requested
⏵ 1	2026-03-20 00:48:41	announced

10.1016/J.TCSW.2026.100172;

10.6019/PXD070373;

submitter keyword: fatty acids, transcription factor, proteomic profile,Neurospora crassa, cell wall, glycans, Galleria mellonella, plasma membrane

Peter Barath
contact affiliation	Institute of Chemistry, SAS, Bratislava, Slovakia
contact email	peter.barath@savba.sk
lab head
Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070373
     1. Label: PRIDE project
     2. Name: Neurospora crassa Δccg8 compromises cell surface integrity and antifungal tolerance: Insights from in vitro and Galleria mellonella studies