PXD070284 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Dissecting the Molecular Crosstalk between O-GlcNAcylation and Phosphorylation via Quantitative Proteomics and Phosphoproteomics |
| Description | In this study, we explored the cross-talk between phosphorylation and O-GlcNAcylation using the pancreatic ductal cell line PANC-1 as a model. Proteome and phosphoproteome changes were measured for cells treated with OSMI-1, a specific inhibitor to O-GlcNAc transferase (OGT) and Thiamet G, a specific inhibitor to O-GlcNAcase (OGA). Among the 8938 phosphorylation sites quantified, 1399 phosphosites on 870 proteins and 1480 phosphosites on 896 proteins were significantly altered by OSMI-1 and TMG treatment, respectively, demonstrating extensive cross-talk between O-GlcNAcylation and phosphorylation. In-depth analysis revealed widespread phosphorylation changes of the kinome and phosphatome, even after a short-term perturbation with inhibitors to O-GlcNAc cycling enzymes. Notably, we identified that phosphorylation of OGA at S364 is specifically mediated by casein kinase 2 alpha (CK2α), which was supported by phosphoproteomic profiling, kinase inhibition experiments, and in vitro kinase assays. These results uncover a regulatory mechanism underlying glycosylation-dependent cellular signaling through the interplay of phosphorylation and O-GlcNAcylation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-28_17:01:12.927.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ci Wu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-04 02:07:25 | ID requested | |
| ⏵ 1 | 2026-06-28 17:01:13 | announced | |
Publication List
| Wu C, Hou C, Wang X, Peng Y, Lin Y, Byers SW, Pei H, Ma J, GlcNAc-Dependent Signaling Via Integrated Proteomics and Phosphoproteomics. ACS Omega, 11(23):34373-34384(2026) [pubmed] |
| 10.1021/acsomega.6c02310; |
Keyword List
| submitter keyword: phosphorylation crosstalk O-GlcNAc |
Contact List
| Junfeng Ma |
|---|
| contact affiliation | Georgetown University |
| contact email | Junfeng.Ma@georgetown.edu |
| lab head | |
| Ci Wu |
|---|
| contact affiliation | Research specialist |
| contact email | wuci1111@126.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD070284 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070284
- Label: PRIDE project
- Name: Dissecting the Molecular Crosstalk between O-GlcNAcylation and Phosphorylation via Quantitative Proteomics and Phosphoproteomics
