PXD070278

PXD070278 is an original dataset announced via ProteomeXchange.

Title	Multiple Evolutionary Mechanisms Underlie the Venom Composition of the Green Lacewing
Description	Venom has independently evolved across many lineages, yet relatively few have been studied in detail, particularly among insects. Of these, Neuroptera (lacewings, antlions and relatives) remain largely unexplored, despite being widespread with agriculturally important groups such as green lacewings. While adults are non-venomous, neuropteran larvae are ferocious predators that use pincer-like mouthparts to inject paralysing and liquefying venom to subdue and consume their prey. Here, we provide a comprehensive investigation of the venom system in Neuroptera by integrating a high-quality genome, long-read transcriptomes spanning all life stages, microCT-reconstruction of venom glands, tissue-specific expression analyses, venom proteomics, and functional assays of the common green lacewing Chrysoperla carnea. We provide a re-description of the neuropteran venom system, demonstrate the venom’s insecticidal and cytotoxic activity, and show the venom comprises diverse toxin gene families and is richer and more similar to the venom of antlions than previously proposed. We show that this toxin arsenal is the result of a multitude of evolutionary events that include co-option, recruitment following gene duplication, diversification of toxin-paralogs by gene duplication, and functional innovation of new paralogs through both small structural and large architectural changes. In addition, we find that alternative splicing of toxin genes is an important contributor to the biochemical arsenal, which is a mechanism rarely documented among venomous animals. Our results demonstrate how multiple genomic and evolutionary mechanisms together contribute to the emergence and evolution of a complex molecular trait, and provide new insights into the evolution of venom in insects.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_17:15:36.241.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marius Maurstad
SpeciesList	scientific name: Chrysoperla carnea; NCBI TaxID: NEWT:189513;
ModificationList	amidated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 23:54:28	ID requested
⏵ 1	2026-01-11 17:15:37	announced

10.1093/molbev/msaf326;

Maurstad MF, Ramiro IBL, Oeyen JP, Sombke A, B, ü, sse S, Nachtigall PG, Jakobsen KS, Undheim EAB, The green lacewing venom system and the complex mechanisms underlying its evolution. Mol Biol Evol, 43(1):(2026) [pubmed]

submitter keyword: Venom

Eivind Andreas Baste Undheim
contact affiliation	Centre for Ecological and Evolutionary Synthesis, Department of Biosciences, University of Oslo, PO Box 1066 Blindern, 0316 Oslo, Norway
contact email	e.a.b.undheim@ibv.uio.no
lab head
Marius Maurstad
contact affiliation	University of Oslo, Department of Biosciences
contact email	m.f.maurstad@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD070278

PRIDE project URI

• PRIDE
  1. PXD070278
     1. Label: PRIDE project
     2. Name: Multiple Evolutionary Mechanisms Underlie the Venom Composition of the Green Lacewing