PXD070258

PXD070258 is an original dataset announced via ProteomeXchange.

Title	MclX modulates the process of mother cell lysis by SpoIIID in Bacillus thuringiensis
Description	Cell differentiation is essential for the survival and adaptation of organisms. In Bacillus species, sporulation is a tightly coordinated program that culminates in mother cell lysis, a critical step for spore release. Despite its importance, the mechanisms modulating the process remain poorly understood. Here, we show that MclX regulates mother cell lysis by inhibiting SpoIIID, a sporulation-specific transcription factor that represses cwlC, which encodes a key hydrolase required for lysis. SpoIIID directly binds to the cwlC promoter, and its overexpression during sporulation markedly delays both the onset and progression of mother cell lysis. These findings support a model in which MclX modulates SpoIIID to control cwlC expression and lysis process. Collectively, this work defines a previously unrecognized MclX–SpoIIID–cwlC regulatory axis, providing new insight into the control of late sporulation events and the process of mother cell lysis.
HostingRepository	PRIDE
AnnounceDate	2026-07-15
AnnouncementXML	Submission_2026-07-14_22:19:09.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yanrong xu
SpeciesList	scientific name: Bacillus thuringiensis; NCBI TaxID: NEWT:1428;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 17:00:23	ID requested
⏵ 1	2026-07-14 22:19:10	announced

Dataset with its publication pending

submitter keyword: Mother cell lysis initiation,Bacillus thuringiensis, Protein of unknown function MclX, Hydrolase gene cwlC, Transcriptional regulator SpoIIID

Yanrong Xu
contact affiliation	South Gate 2, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, China.
contact email	xu_yanrong@outlook.com
lab head
yanrong xu
contact affiliation	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences.
contact email	xu_yanrong@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD070258

PRIDE project URI

• PRIDE
  1. PXD070258
     1. Label: PRIDE project
     2. Name: MclX modulates the process of mother cell lysis by SpoIIID in Bacillus thuringiensis