PXD070207

PXD070207 is an original dataset announced via ProteomeXchange.

Title	PI4KIIα is the palmitoylation reader that directs Golgi-to-plasma membrane trafficking
Description	Protein palmitoylation is a pivotal lipid modification that dictates membrane trafficking specificity. Here, we find phosphatidylinositol 4-kinase IIα (PI4KIIα) is the essential for transport of palmitoylated cargoes from the trans-Golgi network (TGN) to the plasma membrane (PM). Here, we performed quantitative stable isotope labeling by amino acids in cell culture (SILAC) proteomics in wild-type (WT) and PI4K2A-/- cells. Isolated PM fractions were divided for two parallel analyses: one was directly subjected to mass spectrometry, identifying PI4KIIα-dependent PM proteins; the other was first enriched for palmitoylated proteins via Acyl-RAC analysis, identifying PI4KIIα-dependent palmitoylated PM proteins.
HostingRepository	iProX
AnnounceDate	2025-11-25
AnnouncementXML	Submission_2026-08-25_00:46:02.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Langfan Zheng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 00:21:03	ID requested
⏵ 1	2026-08-25 00:46:02	announced

Dataset with its publication pending

submitter keyword: PI4K2A, SILAC, Palmitoylation membrane protein

Tongjin Zhao
contact affiliation	State Key Laboratory of Genetics and Development of Complex Phenotypes, Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Zhongshan Hospital, Fudan University
contact email	zhaotj@fudan.edu.cn
lab head
Langfan Zheng
contact affiliation	Institute of Metabolism and Integrative Biology, Fudan University
contact email	1744660796@qq.com
dataset submitter

iProX dataset URI