PXD070186

PXD070186 is an original dataset announced via ProteomeXchange.

Title	IP-MS Profiling of ANAPC1 and MP1 Interaction Networks in OVCAR3 Cells
Description	We identified a pseudogene-derived chimeric transcript, vA-202, which encodes a partial ANAPC1-derived protein (MP1). To characterize its protein interaction network, we transiently expressed FLAG-tagged MP1 in OVCAR3 cells and performed immunoprecipitation using an anti-FLAG antibody. For comparison with the parental protein, endogenous ANAPC1 was immunoprecipitated using two antibodies: one specific to ANAPC1 alone and another recognizing an epitope shared with MP1. Corresponding IgG and peptide competition controls were included to eliminate nonspecific interactions. Immunoprecipitated protein complexes were subjected to LC-MS/MS analysis to identify interacting partners of both ANAPC1 and MP1.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:32:14.742.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amruta Jadhav
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-01 05:48:16	ID requested
⏵ 1	2026-05-24 16:32:15	announced

10.1093/nar/gkag375;

Jadhav AR, Sahoo S, Kuber YL, Hatwar K, Varanwal A, Tanwar K, Palwankar S, Chatrasal G, Mali AM, Bapat SA, genes) contribute to genetic and functional diversity of the human genome. Nucleic Acids Res, 54(8):(2026) [pubmed]

submitter keyword: Human, MP1, ANAPC1, IP-MS, Ovarian cancer

Dr. Sharmila A. Bapat
contact affiliation	BRIC-National Centre for Cell Science, Inside Pune Univesrity, Ganeshkhind, Pune, India, 411007
contact email	sabapat@nccs.res.in
lab head
Amruta Jadhav
contact affiliation	National centre for cell science, Pune, India
contact email	amrutaj355@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD070186

PRIDE project URI

• PRIDE
  1. PXD070186
     1. Label: PRIDE project
     2. Name: IP-MS Profiling of ANAPC1 and MP1 Interaction Networks in OVCAR3 Cells