⮝ Full datasets listing
PXD070166
PXD070166 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MS-MS analysis of coomassie stained protein bands observed on the Alzheimer model active compound drug resin (102) versus control resin (74) |
| Description | Pig brain was homogenized in column buffer supplemented with protease inhibitors and 10kx/g supernate prepared and flash frozen. Aliquot was thawed, 100ul applied to the 50ul bed volume drug resin (124) or control resin lacking the drug ligand (74) and incubated for 1hr at room temperature followed by 100 bed volumes of wash with column buffer. Free drug at 200uM in column buffer was used to elute bound proteins, and the drug resin stripped with 8M urea to remove proteins that may have stuck after elution. Samples 16-18 were the bands observed in the control resin. Samples 4-14 were the bands observed in the drug resin. Samples 1-3 were the bands observed in the 8M urea stripping of the drug resin and not observed upon similar stripping the control resin. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-03 |
| AnnouncementXML | Submission_2025-11-03_12:00:20.492.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | MS Bioworks |
| SpeciesList | scientific name: Sus scrofa; common name: pig; NCBI TaxID: 9823; |
| ModificationList | Carbamidomethyl |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-31 12:14:52 | ID requested | |
| ⏵ 1 | 2025-11-03 12:00:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Protein assembly modulation, allosteric site, small molecule, pig brain, Alzheimers Disease, DatasetType:Proteomics |
Contact List
| Vishwanath R. Lingappa, MD, PhD | |
|---|---|
| contact affiliation | Prosetta Biosciences, Inc |
| contact email | vlingappa@prosetta.com |
| lab head | |
| MS Bioworks | |
| contact affiliation | MS Bioworks |
| contact email | mford@msbioworks.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099711/ |
