PXD070151

PXD070151 is an original dataset announced via ProteomeXchange.

Title	UPS1 and UPS2 TMT Benchmarking Experiment
Description	This experiment is a TMT10plex labelled experiment containing trypsin digested UPS1 and UPS2 human proteins in replicate along with E.Coli lysates. The mass spectra were generated in a single MS2-based experiment, using TMT10plex-labelled samples made up of UPS1, UPS2, and E.Coli lysate. UPS1 (Universal Proteomics Standard) and UPS2 (Proteomics Dynamic Range Standard) are publicly available reference standards, used in mass spectrometry (supplied by Sigma-Aldrich) [33]. Both consist of the same 48 recombinant human proteins (ranging from 6,000Da to 83,000Da in mass), present in equal abundance in UPS1 and at different abundances in UPS2 (from 500 amoles to 50 pmoles).
HostingRepository	PRIDE
AnnounceDate	2026-05-15
AnnouncementXML	Submission_2026-05-15_01:52:26.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-31 06:31:27	ID requested
⏵ 1	2026-05-15 01:52:27	announced

Dataset with its publication pending

submitter keyword: Human, MS/MS, UPS, E.Coli

Jyoti Choudhary
contact affiliation	The Institute of Cancer Research, London
contact email	jyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliation	The Institute of Cancer Research
contact email	james.wright@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070151
     1. Label: PRIDE project
     2. Name: UPS1 and UPS2 TMT Benchmarking Experiment