PXD070142

PXD070142 is an original dataset announced via ProteomeXchange.

Title	Pathways to spontaneous immortalization: proteome changes underlying the transition from primary cultures to fish cell lines
Description	Quantitative proteomics identified passage 4 (P4) as a pivot from transient surges to durable, post-crisis proliferation of two independently derived L. tenuis embryonic cell cultures (LtE-1 and LtE-2). A shift from expression of fibroblast to epithelial protein markers occurred around the crisis pivot. In LtE-1, protein-synthesis functions peaked pre-P4, reversed at P5, and were followed by balancing proteostasis. Cell-fate signaling (MAPK, AKT, WNT, NF-κB) spiked immediately after P4. In LtE-2, crisis occurred slightly later and upregulated RNA processing and translation was observed until P5, followed by a reversal at P6, and balancing of proteostasis at P7. The regulation of proteostasis was accompanied by abundance changes of structural and ECM proteins that could contribute to the post-crisis shift towards epithelial cell morphology. Network analysis of proteome regulation in LtE-1 contrasted pre-P4 ribosome-centered networks with post-P4 proteome networks reflecting regulation of sterol/cholesterol biosynthesis, ECM/collagen, and cytoskeletal functions.
HostingRepository	PanoramaPublic
AnnounceDate	2026-06-17
AnnouncementXML	Submission_2026-06-17_18:43:40.769.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dietmar Kültz
SpeciesList	scientific name: Leuresthes tenuis; NCBI TaxID: 355514;
ModificationList	Acetyl; Carbamidomethyl; Oxidation
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-31 01:06:39	ID requested
⏵ 1	2026-06-17 18:43:42	announced

Corona MM, Li J, K, ü, ltz D, Proteome dynamics during establishment of California grunion (Leuresthes tenuis) cell lines. BMC Biol, 24(1):(2026) [pubmed]

submitter keyword: DIA proteomics, immortalization, senescence, cell line establishment, fish cell line, cell culture crisis

Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
dataset submitter

Panorama Public dataset URI