PXD070131 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The proteome of acute muscle pain: Observations from acute hypertonic saline induced pain in humans |
| Description | Despite widespread use of experimental acute pain models to mimic chronic pathologies, little exploration has been undertaken on the acute pain proteome in humans. We re-solved to explore molecular alterations evoked by acute bouts of hypertonic saline (HS) induced muscle pain and map the spread of evoked mechanical hyperalgesia. This study used a 2-cohort design in healthy human participants. Cohort one (n=16) underwent in-termittent blood sampling concurrent with intramuscular HS (5%) infusion to allow for the discovery of the proteomic and cytokine profile of acute muscle pain. Cohort two (n=10) underwent bilateral sensory testing during HS infusion, to map the spread of evoked mechanical hyperalgesia. Molecular analysis in cohort one revealed a diffuse ar-ray of proteins and cytokines showing altered expression in response to acute muscle pain. Particularly these alterations were linked to metabolism and immune response pathways suggestive of systemic effects of acute pain. Intriguingly, cohort two revealed a significant mechanical hyperalgesia which emerged in a diffuse pattern over the ipsilat-eral limb to HS-infusion. However, despite evidence of systemic molecular alterations, no such mechanical hypersensitivity was observed in the contralateral limb. This study demonstrates systemic molecular alterations resultant from acute bouts of HS-induced muscle pain with accompanying sensory interactions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-06 |
| AnnouncementXML | Submission_2026-01-06_11:31:25.974.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pauline Jubin |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-30 22:17:36 | ID requested | |
| ⏵ 1 | 2026-01-06 11:31:26 | announced | |
Publication List
| Jubin P, Amigo M, Boulton D, Mahns DA, Nagi SS, Dunn JS, The Proteome of Acute Muscle Pain: Observations from Acute Hypertonic-Saline-Induced Pain in Humans. Int J Mol Sci, 26(24):(2025) [pubmed] |
| 10.3390/ijms262411922; |
Keyword List
| submitter keyword: Human, untargeted proteomics, acute pain, nociception |
Contact List
| David A. Mahns |
|---|
| contact affiliation | School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia |
| contact email | d.mahns@westernsydney.edu.au |
| lab head | |
| Pauline Jubin |
|---|
| contact affiliation | Western Sydney University |
| contact email | p.jubin@westernsydney.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD070131 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070131
- Label: PRIDE project
- Name: The proteome of acute muscle pain: Observations from acute hypertonic saline induced pain in humans
