PXD070124

PXD070124 is an original dataset announced via ProteomeXchange.

Title	Reversal of protein aging by enzymatic deglycation
Description	A hallmark of mammalian aging is the accumulation of irreversible molecular damage on proteins of the extracellular matrix. The advanced glycation endproduct Nε-carboxymethyl-lysine (CML) is abundant in aging human tissues, where it generates neoepitopes leading to inflammatory signaling. Here we develop CMLase, a new-to-nature enzyme engineered by testing more than 500 million enzyme variants using a novel genetic selection. We show that CMLase reverses CML modifications across diverse protein substrates, as well as human tissue from elderly donors, demonstrating for the first time that protein damage previously thought to be irreversible can be enzymatically repaired. This approach establishes a platform for developing enzymes to reverse age-related molecular damage and repair proteins compromised by aging or disease.
HostingRepository	PRIDE
AnnounceDate	2026-07-16
AnnouncementXML	Submission_2026-07-16_09:17:27.993.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niclas Olsson
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	N6-carboxymethyl-L-lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-30 17:32:53	ID requested
⏵ 1	2026-07-16 09:17:30	announced

10.1038/s41467-026-75141-2;

Trabosh N, Smith J, Hsu MY, Panja S, Nagaraj R, Olsson N, McAllister FE, Cravens A, Reversal of protein chemical aging by enzymatic deglycation. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: LC-MSMS, CML,Glycation, BSA

Fiona McAllister
contact affiliation	Calico Life Sciences
contact email	fiona@calicolabs.com
lab head
Niclas Olsson
contact affiliation	Calico Life Sciences
contact email	niclas@calicolabs.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD070124

PRIDE project URI

• PRIDE
  1. PXD070124
     1. Label: PRIDE project
     2. Name: Reversal of protein aging by enzymatic deglycation