PXD070115 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Thio-triazole-biotin-labeled peptides derived from iPA probes |
| Description | iPAs are minimal-tag chemical probes - propargyl analogs of putrescine (PUT), spermidine (SPD), and spermine (SPM) - that preserve charge/spacing, retain transporter compatibility, and enable high-resolution intracellular imaging following paraformaldehyde (PFA)-based crosslinking and copper-catalyzed azide-alkyne cycloaddition to a fluorescent reporter. The LC-MS/MS data submitted here support an experiment designed to assess whether intracellular iPAs undergo substantial metabolic/catabolic transformation. MCF-7 cells were treated with iPAs, lysed, and subjected to a copper-catalyzed azide-alkyne-thiol reaction in which solvent-exposed cysteines in proteins react simultaneously with alkyne-bearing probe(s) released from cells and with azide-PEG3-biotin added to the lysis buffer. Proteins were then digested with trypsin; thio-triazole-biotin-labeled peptides were enriched and analyzed by LC–MS/MS. Open-search analysis showed that the observed cysteine mass shifts predominantly correspond to incorporation of the intact, alkyne-retaining probe species. Note that this assay selectively detects alkyne-retaining species and does not quantify potential alkyne-loss products. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-16 |
| AnnouncementXML | Submission_2025-12-15_23:51:06.440.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Remigiusz Serwa |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-30 14:29:15 | ID requested | |
| ⏵ 1 | 2025-12-15 23:51:06 | announced | |
Publication List
Keyword List
| submitter keyword: artificialy modified peptides, Propargyl tag,Polyamines |
Contact List
| Remigiusz Serwa |
|---|
| contact affiliation | IMol, Polish Academy of Sciences, 02-247 Warsaw, Poland |
| contact email | r.serwa@imol.institute |
| lab head | |
| Remigiusz Serwa |
|---|
| contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
| contact email | r.serwa@imol.edu.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070115 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070115
- Label: PRIDE project
- Name: Thio-triazole-biotin-labeled peptides derived from iPA probes
