⮝ Full datasets listing
PXD070035
PXD070035 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MitoSAM-dependent lipoylation controls postnatal heart development via metabolic remodeling |
| Description | The neonatal heart undergoes a rapid metabolic transition from fetal glycolysis to oxidative phosphorylation, requiring coordinated remodeling of mitochondrial metabolism. The mechanisms driving this transition remain unclear. Here, we demonstrate that sufficient mitochondrial S-adenosylmethionine (mitoSAM), imported via the solute carrier Slc25a26, is essential for this shift by sustaining the lipoylation of 2-oxoacid dehydrogenases, critical for tricarboxylic acid (TCA) cycle activation. Proteomic and metabolomic profiling revealed that reduced mitoSAM availability impaired lipoylation, blocking TCA cycle function and restricting nucleotide synthesis, while mitochondrial gene expression and respiratory capacity remained largely intact. In vivo EdU labeling showed persistent cardiomyocyte proliferation imposing further strain on nucleotide pools. Supplementation with medium-chain triglycerides during the suckling-to-weaning transition restored metabolic function and improved cardiac physiology, revealing a critical developmental window in which mitoSAM-dependent lipoylation ensures heart maturation. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-06-22 |
| AnnouncementXML | Submission_2026-06-22_07:25:59.689.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mahshid Zarrineh |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-29 08:18:53 | ID requested | |
| ⏵ 1 | 2026-06-22 07:26:00 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cardiomyocytes, mouse, mitochondria, S-Adenosylmethionine |
Contact List
| Prof Janne Lehtiö | |
|---|---|
| lab head | |
| Mahshid Zarrineh | |
| contact affiliation | Scilifelab, Karolinska institute |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004144/ |
