PXD070016 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Reverse Genetics-Rescued Virulent Feline Calicivirus FCV-BJ616 Recapitulates Systemic Disease and Cytokine Storm in Cats |
| Description | Feline calicivirus (FCV) is a key pathogen causing upper respiratory diseases and oral ulcers in cats, with significant genetic variability. This study aimed to isolate the FCV-BJ616 strain, investigate its pathogenic mechanisms, and provide insights for developing antibody therapies and broad-spectrum vaccines. The virus was purified through three rounds of plaque cloning, and its morphology was observed via electron microscopy. Immunofluorescence and Western blotting confirmed VP1 protein expression. Using systems biology and reverse genetics, an infectious clone of rFCV-BJ616 was constructed and recovered. The recombinant virus maintained genetic stability similar to the parental strain. Animal experiments showed that rFCV-BJ616 exhibited wild-type virulence, causing persistent high fever, weight loss, and multi-organ pathology in infected cats. Proteomic analysis revealed that FCV activates cytokine pathways, causing elevatedIL-8 and IFN-βlevels, leading to a cytokine storm. This study enhances understanding of FCV-BJ616's genetic evolution and successfully established a reverse genetics platform for FCV, enabling precise manipulation of the virus at the genomic level and providing a controllable and reproducible experimental basis for systematic studies on its replication, pathogenesis, and vaccine attenuation mechanisms. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-02 |
| AnnouncementXML | Submission_2026-02-01_16:39:54.798.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | chunmei xu |
| SpeciesList | scientific name: Felis chaus; NCBI TaxID: NEWT:61376; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-29 01:37:07 | ID requested | |
| ⏵ 1 | 2026-02-01 16:39:55 | announced | |
Publication List
| 10.1002/mbo3.70226; |
| Xu C, Zhao J, Liu H, Gu H, Tang X, Liang L, Ding J, Hou S, Zhao X, Liang R, Construction of Reverse Genetics System for Feline Calicivirus FCV-BJ616 and Proteomic Analysis. Microbiologyopen, 15(1):e70226(2026) [pubmed] |
Keyword List
| submitter keyword: Proteomics.,Feline calicivirus, Reverse genetics of viruses |
Contact List
| Chunmei Xu |
|---|
| contact affiliation | No. 2 West Yuanmingyuan Road, Haidian District, Beijing |
| contact email | xcm13592563704@163.com |
| lab head | |
| chunmei xu |
|---|
| contact affiliation | Key Laboratory of Animal Biosafety Risk Prevention and Control (North) and Key Laboratory of Veterinary Biological Products and Chemical Drugs of Ministry of Agriculture and Rural Affairs (MARA), Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. |
| contact email | xcm13592563704@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070016
- Label: PRIDE project
- Name: Reverse Genetics-Rescued Virulent Feline Calicivirus FCV-BJ616 Recapitulates Systemic Disease and Cytokine Storm in Cats
