PXD070009

PXD070009 is an original dataset announced via ProteomeXchange.

Title	Bacteroides acidifaciens derived succinic acid mediates polysaccharides from GPMP induced muscle fiber switching and exercise endurance enhancement
Description	Enhancing exercise endurance holds significant practical implications for boosting the physical fitness of the general population to address daily challenges and improving the physical capabilities and quality of life of patients with relevant diseases. Polysaccharides from Gynostemma pentaphyllum Makino (GPMP) have been shown to increase the expression of slow muscle fibers in muscles. However, the underlying mechanism remains to be further investigated. Previous research has indicated that alterations in the gut microbiota are closely intertwined with the improvement of muscle endurance. In this study, we demonstrate that GPMP enriches B. acidifaciens in the murine gut, showing a notable association with muscle endurance. Subsequently, we identified that succinic acid, a key metabolite of B. acidifaciens, can effectively enhance the exercise endurance of mice and the antioxidant capacity of their muscles, protecting the muscles from damage induced by strenuous exercise. Specifically, succinic acid enters the circulatory system and acts upon skeletal muscles, activating the non-canonical Wnt signaling pathway. This activation leads to an increase in slow muscle fibers and an elevation of the oxidative phosphorylation level in muscle tissues, ultimately contributing to the improvement of exercise endurance. Moreover, we observed similar increases in B. acidifaciens and succinic acid in the feces and sera of endurance athletes. Overall, these findings uncover that GPMP modulates the body's exercise endurance through specific gut microbiota and their metabolites, thereby establishing a regulatory mechanism of host exercise endurance by gut microbiota.
HostingRepository	iProX
AnnounceDate	2025-10-28
AnnouncementXML	Submission_2025-10-28_23:41:47.182.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junqing Su
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-28 23:40:59	ID requested
⏵ 1	2025-10-28 23:41:47	announced

Dataset with its publication pending

submitter keyword: gut microbiota;muscle

Xiaoya Shang
contact affiliation	Northwestern Polytechnical University
contact email	shangxy@nwpu.edu.cn
lab head
Junqing Su
contact affiliation	Northwestern Polytechnical University
contact email	sujunqing@mail.nwpu.edu.cn
dataset submitter

iProX dataset URI