PXD070005 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Guard Cell-Enriched Phosphoproteome from open and closed stomata |
| Description | Control of the stomatal aperture is multifaceted, involving a complex interplay of environmental cues and intracellular signaling pathways. It is well established that changes in ion gradients drive water movement into and out of the guard cell, thereby altering cell volume and modulating the opening or closing of the stomatal pore. These rapid responses are often regulated by phosphorylation cascades to efficiently transmit environmental status and either reduce water loss or enhance carbon assimilation. Here, we investigated the regulation of stomatal opening and closing by generating a proteome and phosphoproteome of guard cell-enriched tissue. The guard cell-enriched tissue with closed stomata showed greater levels of phosphorylation of proteins related to endomembrane trafficking and vacuoles when compared to both whole leaf tissue with closed stomata and guard cell-enriched tissue with open stomata. These results support the hypothesis that phosphorylation of endomembrane proteins may contribute to the regulation of stomatal movements. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-22 |
| AnnouncementXML | Submission_2026-05-22_10:56:11.502.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Scott Lyons |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-28 22:36:33 | ID requested | |
| ⏵ 1 | 2026-05-22 10:56:12 | announced | |
Publication List
Keyword List
| submitter keyword: guard cell,plant, phosphorylation, endomembrane system, stomata, vacuole |
Contact List
| Marcela Rojas-Pierce |
|---|
| contact affiliation | Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27514, USA |
| contact email | mrojasp@ncsu.edu |
| lab head | |
| Scott Lyons |
|---|
| contact affiliation | UNC Chapel Hill |
| contact email | scott_lyons@med.unc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD070005 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070005
- Label: PRIDE project
- Name: Guard Cell-Enriched Phosphoproteome from open and closed stomata
