PXD069971

PXD069971 is an original dataset announced via ProteomeXchange.

Title	TurboID-based proximity labeling identifies PsbS neighboring proteins in Arabidopsis thaliana leaf chloroplasts under high light
Description	Photosystem Ⅱ Subunit S (PsbS) is a critical regulator of non-photochemical quenching (NPQ), which is a protective mechanism triggered to dissipate excess light energy as heat and prevent photodamage. However, the molecular basis of how PsbS interact with partner proteins to regulate NPQ remains unclear. In this study, we employed proximity labeling to identify PsbS interaction proteins in situ in living cells of Arabidopsis leaves via biotinylation during NPQ. Arabidopsis plants stably expressing PsbS constructs fused to proximity labeling enzyme TurboID were generated and the biotinylated proteomes were analyzed by liquid chromatography-mass spectrometry. The interactomes of PsbS under dark and under light were generated, which not only confirmed several known PsbS-interacting proteins, such as Lhcb1.3, Lhcb3, and Lhcb4.2, but also identified many novel binding proteins. Interestingly, most of these protein interactions of PsbS were unaffected by light, which suggest that the PsbS might influence the NPQ through conformational changes without a large physical migration within thylakoid membrane. Analyses of the interactomes also show a few proteins enhanced (such as TLP18.3) or some proteins inhibited (such as ZEP) under the high light, suggesting that the NPQ and repair process after photoinhibition might be coordinated.
HostingRepository	iProX
AnnounceDate	2025-10-28
AnnouncementXML	Submission_2025-10-28_01:41:23.619.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhongtian Zhao
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-28 01:41:03	ID requested
⏵ 1	2025-10-28 01:41:24	announced

Dataset with its publication pending

submitter keyword: PsbS, Proximity labeling proteomics, Photosynthesis, NPQ, Arabidopsis thaliana.

Huiqiong Zheng
contact affiliation	Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences
contact email	hqzheng@cemps.ac.cn
lab head
Zhongtian Zhao
contact affiliation	Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences
contact email	zhaozhongtian@cemps.ac.cn
dataset submitter

iProX dataset URI