PXD069965

PXD069965 is an original dataset announced via ProteomeXchange.

Title	Mouse TAPBPR functions as an MHC-I peptide exchange catalyst
Description	Human TAPBPR is known to function as a Major Histocompatibility Complex class I (MHC-I) peptide exchange catalyst which shapes the peptide repertoire presented to immune cells. Never14theless, investigations characterizing TAPBPR from other species are limited. Here, we characterize mouse TAPBPR, exploring its association partners in mouse cell lines and comparing its function to human TAPBPR. We find that mouse TAPBPR binds MHC-I and calnexin, with a notably sustained interaction with H2-Db compared to H2-Kb. We reveal mouse TAPBPR mediates peptide exchange on both H2-Db and H2-Kb, restricting the peptide repertoire presented on MC-38 cells. Intriguingly, mouse TAPBPR promotes the selection of peptides with a C-terminal methionine on H2-Kb. Together, our findings establish that mouse TAPBPR plays an important role in shaping the MHC-I immunopeptidome by functioning as a peptide editor, like its human counterpart.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_06:46:10.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcel Wacker
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-28 00:19:24	ID requested
⏵ 1	2026-03-23 06:46:11	announced

Dataset with its publication pending

submitter keyword: Mouse, TAPBPR, Immunopeptidomics, MHC-I, LC-MS/MS

Dr. Juliane Sarah Walz
contact affiliation	Department of Peptide-based Immunotherapy, Institute of Immunology, University and University Hospital Tübingen, Tübingen, BW, Germany
contact email	Juliane.Walz@med.uni-tuebingen.de
lab head
Marcel Wacker
contact affiliation	Department of Peptide-based Immunotherapy, University and University Hospital Tübingen, Tübingen, Germany
contact email	marcel.wacker@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069965
     1. Label: PRIDE project
     2. Name: Mouse TAPBPR functions as an MHC-I peptide exchange catalyst