PXD069890 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cannabinoid Receptor Type 2 Agonist JWH-133 Stimulates Antiviral Factors and Decreases Proviral, Inflammatory, and Neurotoxic Proteins in HIV-Infected Macrophage Secretome |
| Description | Although antiviral therapy has improved quality of life, around 50% of people with HIV (PWH) experience neurodegeneration and cognitive decline. This is prompted in part by migration of HIV-infected monocyte-derived macrophages (MDM) to the brain, leading to neuronal death. Previous studies in our lab have shown that HIV-infected MDM secrete cathepsin B (CATB), which is a pro-inflammatory neurotoxic enzyme that is reduced by the addition of cannabinoid receptor-2 (CB2R) agonist JWH-133 to cell cultures. In this study, we aimed to identify the secreted proteins (secretome) by HIV-infected macro-phages exposed to JWH-133 and quantify them using Tandem Mass Tag (TMT) mass spectrometry. Frozen 13-day MDM supernatants from: (1) MDM negative control; (2) HIV-MDM; and (3) MDM-HIV-JWH-133, were compared in triplicates by mass spectrometry (LC/MS/MS) and analyzed for protein identification. Subsequently, the same samples were labeled by TMT labeling and quantified by LC/MS/MS. After database search, 528 proteins were identified from all groups. Thereafter, proteins with more than 3 unique peptides and more than 10% coverage were selected for protein ID. Venn diagrams revealed 1 unique protein secreted by MDM-HIV, 10 unique proteins in MDM-HIV JWH-133, and 15 common proteins in the three groups. CATB was unique to MDM-HIV. MDM-HIV exposed to JWH-133 showed proteins related to metabolism, cell organization, anti-viral activity, and stress response. TMT analysis revealed 1,454 proteins with abundance for statistical analysis based on FC ≥ |1.5| and p-value ≤ 0.05, of which Ruvb-like 1 and Hornerin decreased significantly with JWH-133 treatment. Both proteins stimulate HIV replication. In addition, HIV infection upregulated proteins associated with pathways of HIV latency that were inhibited by JWH-133. In conclusion, JWH-133 treatment in HIV-infected macrophages leads to the secretion of antiviral host factors and decreases the secretion of proviral, inflammatory, and neurotoxic host factors. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-24 |
| AnnouncementXML | Submission_2025-11-23_16:22:56.426.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD069890 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Loyda Melendez |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-24 14:43:25 | ID requested | |
| ⏵ 1 | 2025-11-23 16:22:57 | announced | |
Publication List
| 10.6019/PXD069890; |
| Rosario-Rodr, í, guez LJ, Cantres-Rosario YM, Rodr, í, guez De Jes, ú, s AE, Mera-P, é, rez AM, Tosado-Rodr, í, guez EL, Roche Lima A, Mel, é, ndez LM, Cannabinoid Receptor Type 2 Agonist JWH-133 Stimulates Antiviral Factors and Decreases Proviral, Inflammatory, and Neurotoxic Proteins in HIV-Infected Macrophage Secretome. Int J Mol Sci, 26(21):(2025) [pubmed] |
| 10.3390/ijms262110596; |
Keyword List
| submitter keyword: HIV-associated neurocognitive disorders |
| CB2R |
| JWH-133 |
| secretome |
| LC/MS/MS |
| TMT |
| proteomics |
Contact List
| Loyda Melendez |
|---|
| contact affiliation | Translational Proteomics Center, RCMI Program, Department of Microbiology, University of Puerto Rico, Medical Sciences Campus |
| contact email | lmelendezlab@gmail.com |
| lab head | |
| Loyda Melendez |
|---|
| contact affiliation | University of Puerto Rico Medical Sciences Campus |
| contact email | lmelendezlab@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069890
- Label: PRIDE project
- Name: Cannabinoid Receptor Type 2 Agonist JWH-133 Stimulates Antiviral Factors and Decreases Proviral, Inflammatory, and Neurotoxic Proteins in HIV-Infected Macrophage Secretome
