PXD069883

PXD069883 is an original dataset announced via ProteomeXchange.

Title	The proteomics analysis of mitochondria-enriched fractions reveals alteration of key mitochondrial pathways in breast cancer cell lines
Description	Breast cancer is the leading cause of death in women worldwide. Plasticity and heterogeneity of breast cancer cells make this disease extremely difficult to treat. Many reports point to metabolic shifts, mainly those carried out in o mitochondria, as key processes governing the behavior and heterogeneity of several types of breast cancer. In this study, we performed label-free proteomics analysis on mitochondria-enriched fractions from T47D and MDAMB-231 breast cancer cell lines, which have distinct molecular classifications, using bioinformatics analyses to identify differentially expressed proteins compared to MCF-12F healthy breast cells. Cancer cells exhibited down-regulated protein levels of subunits from the respiratory chain's Complex I. However, both presented differentially abundant proteins related to ligase and oxidoreductase activities, such as enzymes involved in glycolysis, pyruvate metabolism, the Krebs cycle, and gluconeogenesis. Many of these enzymes also participate in other metabolic processes, such as localization to the mitochondrion, mitochondrial gene expression, and the metabolism of amino acids, fatty acids, purines, and pyrimidines. Gene Set Enrichment Analysis revealed that OXPHOS enzymes play key roles in many neurodegenerative disease pathways. A protein set with little or no evidence in breast cancer was identified, which could lead to future research in breast cancer mitochondrial metabolism.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-27_04:04:51.756.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rodolfo Moreno Castillo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	amidated residue; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-24 08:26:59	ID requested
⏵ 1	2026-04-27 04:04:53	announced

10.1016/j.jprot.2026.105661;

V, á, zquez-Narv, á, ez HD, Labra-Barrios ML, R, í, os-Castro E, Casta, ñ, ó, n-S, á, nchez CA, Uribe-Carvajal S, Hern, á, ndez-Mart, í, nez E, Hern, á, ndez-Hern, á, ndez JM, Le, ó, n-, Á, vila G, P, é, rez-Rangel A, Á, ngeles-Morales EB, Luna-Arias JP, The proteomics analysis of mitochondria-enriched fractions reveals alteration of key mitochondrial pathways in breast cancer cell lines. J Proteomics, 328():105661(2026) [pubmed]

submitter keyword: T47D and MDA-MB-231 breast cancer cell lines

healthy breast MCF-12F cell line

mitochondria

differentially expressed proteins

label-free proteomics analysis

Juan Pedro Luna-Arias
contact affiliation	Departamento de Biología Celular. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional.Avenida Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Ciudad de México, Código Postal 07360.
contact email	jpluna@cinvestav.mx
lab head
Rodolfo Moreno Castillo
contact affiliation	Instituto Politécnico Nacional
contact email	rmorenoc1700@alumno.ipn.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069883

PRIDE project URI

• PRIDE
  1. PXD069883
     1. Label: PRIDE project
     2. Name: The proteomics analysis of mitochondria-enriched fractions reveals alteration of key mitochondrial pathways in breast cancer cell lines