PXD069882

PXD069882 is an original dataset announced via ProteomeXchange.

Title	C2C12 PEA and Ibuprofen LC-MS/MS
Description	This study investigated the effects of palmitoylethanolamide (PEA) and ibuprofen (IBU) treatment on the dynamic regulation of the skeletal muscle proteome using a C2C12 myotube model. Differentiated myotubes were treated for 36 hours with vehicle control (VC), PEA (10 µM), or IBU (100 µM). Dynamic proteome profiling was performed using deuterium oxide (D₂O) metabolic labelling combined with high-resolution LC–MS/MS analysis on an Orbitrap Q-Exactive (QE) instrument. Samples were collected at 0 h, 12 h, 24 h, and 36 h, with n = 3 biological replicates per timepoint and condition. Protein-specific fractional synthesis rates (FSR) were derived from the 12–36 h labelling period, and relative protein abundance was quantified at 36 h.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_23:03:05.711.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD069882
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Connor Stead
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-24 08:06:42	ID requested
⏵ 1	2026-05-24 23:03:06	announced

10.6019/PXD069882;

10.1096/fba.2025-00286;

Cole PL, Stead CA, Burniston JG, Owens DJ, The Effects of Palmitoylethanolamide or Ibuprofen on the Abundance Profile and Synthesis Rate of Proteins in C2C12 Skeletal Myotubes. FASEB Bioadv, 8(4):e70108(2026) [pubmed]

submitter keyword: C2C12, Muscle, LCMS/MS

Dan Owens
contact affiliation	Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, United Kingdom
contact email	d.j.owens@ljmu.ac.uk
lab head
Connor Stead
contact affiliation	Liverpool John Moores University
contact email	c.stead@ljmu.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD069882

PRIDE project URI

• PRIDE
  1. PXD069882
     1. Label: PRIDE project
     2. Name: C2C12 PEA and Ibuprofen LC-MS/MS