PXD069843

PXD069843 is an original dataset announced via ProteomeXchange.

Title	Identification of AURORA A interaction partners
Description	The oncogenic kinase AURORA A is essential for mitotic progression, and its catalytic inhibition arrests cells at the G2/M-transition. Unexpectedly, partial degradation of AURORA A by PROTACs (proteolysis targeting chimeras) induces profound S-phase defects, revealing a non-catalytic scaffolding function of AURORA A during interface. To dissect this function, we profiled the AURORA A S-phase interactome and identified multiple RNA-binding proteins not known as canonical AURORA A substrates. Among these, the ribonuclease DICER directly associates with AURORA A to form an abundant nuclear complex. RNA degradation shifted AURORA A, DICER, and additional RNA-binding proteins from heavy to light gradient fractions, implicating RNA-dependent complex function. In contrast, PROTAC-mediated depletion of AURORA A altered the gradient migration behavior and chromatin association of the histone methyltransferase SETD2. These findings reveal a dual-output model for the S-phase AURORA A complex: First, RNA-binding proteins are recruited to R-loops, which may arise from transcription-replication conflicts. DICER then processes the R-loop, while AURORA A simultaneously recruits SETD2. This coordinated action prevents the stressed region from being disrupted by spurious transcription, thereby facilitating efficient resolution of replicative stress.
HostingRepository	PRIDE
AnnounceDate	2026-06-03
AnnouncementXML	Submission_2026-06-03_08:32:57.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schlosser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-23 13:51:46	ID requested
⏵ 1	2026-06-03 08:32:57	announced

Dataset with its publication pending

submitter keyword: coIP, interactome

Andreas Schlosser
contact affiliation	Rudolf Virchow Center University of Wuerzburg
contact email	andreas.schlosser@uni-wuerzburg.de
lab head
Andreas Schlosser
contact affiliation	Mass Spectrometry and Proteomics
contact email	andreas.schlosser@virchow.uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069843
     1. Label: PRIDE project
     2. Name: Identification of AURORA A interaction partners