PXD069839

PXD069839 is an original dataset announced via ProteomeXchange.

Title	Characterization of post-mitotic DNA synthesis and its regulation in human cells
Description	DNA replication stress promotes cancer development and is associated with DNA synthesis beyond S-phase. Replication stress-induced DNA lesions and under-replicated DNA can persist into G2 and mitosis, where they undergo mitotic DNA synthesis (MiDAS). After cell division, inherited DNA lesions are protected by genome caretaker proteins and show signs of post-mitotic DNA synthesis (post-MiDAS) in G1. The genomic regions that undergo post-MiDAS, the cellular factors involved, and the implications for genome integrity remain poorly understood. Here, we provide evidence for post-MiDAS activity at centromeres and telomeres in cancer cells employing alternative lengthening of telomeres (ALT) and present data suggesting that a subset of these fragile genomic regions can cluster during post-MiDAS in G1. We further show that post-MiDAS is promoted by local SUMOylation and BLM recruitment into ALT-associated PML bodies (APBs) and involves DNA damage tolerance factors and proteins associated with ALT and MiDAS. Consistent with G1-specific regulation, complementary quantitative proteomics and targeted siRNA screening reveal that APC/C, together with its G1 co-activator CDH1 but independent of its mitotic co-activator CDC20, restrains excessive post-MiDAS in G1. Finally, we show that CDH1 depletion selectively impairs the survival of ALT-positive cancer cell lines, indicating that post-MiDAS might represent a potential ALT-specific vulnerability. This dataset contains TurboID-RPA32 proximity labeling mass spectrometry data generated from FACS-sorted G1-phase U-2 OS FUCCI TurboID-RPA32-HA cells to identify proteins in proximity to RPA32.
HostingRepository	PRIDE
AnnounceDate	2026-08-26
AnnouncementXML	Submission_2026-08-26_02:42:02.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Vornberger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-23 13:25:17	ID requested
⏵ 1	2026-08-26 02:42:03	announced

Dataset with its publication pending

submitter keyword: None

Matthias Altmeyer
contact affiliation	Vetsuisse Faculty (VET), Department of Molecular Mechanisms of Disease, University of Zurich (UZH)
contact email	matthias.altmeyer@uzh.ch
lab head
Julia Vornberger
contact affiliation	Department of Molecular Mechanisms of Disease (DMMD) University of Zurich (UZH)
contact email	julia.vornberger@dmmd.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD069839

PRIDE project URI

• PRIDE
  1. PXD069839
     1. Label: PRIDE project
     2. Name: Characterization of post-mitotic DNA synthesis and its regulation in human cells