PXD069815

PXD069815 is an original dataset announced via ProteomeXchange.

Title	Coronavirus nucleocapsid manipulates mitophagy to restrain innate immunity via SQOR blockade and non-canonical ILF3 degradation
Description	The proteomic analysis were conducted by Majgrbi, Biotech (Shanghai, China). Totalprotein was extracted from lung tissues of mock and IBV infected chickens, usingprotein lysate containing protease inhibitors according to manufacturer's instructions.Protein samples were alkylated and digested by trypsin to prepared peptides. Afterdesalination and quantification, peptides were analyzed by an EASY nLC-1200 system(Thermo, USA) coupled with a timsTOE Pro2 (Bruker, Germany) mass spectrometer.MS/MS spectra were searched using MaxQuant software(version 2.0.3.1)Bioinformatic analysis of proteomic data was performed with the Majgrbig Cloudplatform (https://cloud.majorbio.com). Differential expression analysis betweensamples was performed using DESeq2. The differentially expressed proteins (DEPs)were defined as fold change (>1.5 or < 0.67) and p adjust < 0.05. Enrichment analysisfor the functions of DEPs was conducted based on Kyoto Encyclopedia of Genes andGenomes (KEGG) datasets. Gene set enrichment analysis (GSEA) was performed withGSEA PreRanked tool.
HostingRepository	iProX
AnnounceDate	2025-10-23
AnnouncementXML	Submission_2025-10-23_01:09:15.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Ge
SpeciesList	scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-23 01:09:00	ID requested
⏵ 1	2025-10-23 01:09:15	announced

Dataset with its publication pending

submitter keyword: Coronavirus mitophagy innate immunity

Jiyong Zhou
contact affiliation	College of Animal Sciences Zhejiang University
contact email	jyzhou@zju.edu.cn
lab head
Yao Ge
contact affiliation	College of Animal Sciences Zhejiang University
contact email	1330188852@qq.com
dataset submitter

iProX dataset URI