PXD069807

PXD069807 is an original dataset announced via ProteomeXchange.

Title	Proteomic dataset of MECP2-deficient and wild-type human brain organoids under spaceflight and ground conditions
Description	This dataset presents deep proteomic profiles of human brain organoids cultured aboard the International Space Station (ISS) and on Earth. We analyzed MECP2-deficient (Q83X) and isogenic wild-type control (WT83) organoids exposed to spaceflight and ground conditions for 30 days. Using LC-MS/MS on an Orbitrap Astral mass spectrometer, we confidently identified 56,639 peptides mapping to approximately 6,000 protein groups. The Q83X line models Rett syndrome, carrying a nonsense mutation that produces a severely truncated MeCP2 protein lacking critical functional domains. This unique dataset enables investigation of how spaceflight environment and MECP2 deficiency interact to affect human neural development, offering applications for understanding astronaut health risks and neurodevelopmental disorder mechanisms.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-09_16:00:02.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulo Carvalho
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 19:25:57	ID requested
⏵ 1	2026-02-09 16:00:02	announced

Dataset with its publication pending

submitter keyword: astrocytes, proteomics,brain organoids, L1 retrotransposition, MECP2, microgravity, retrotransposons, neurodegeneration, senescence

ALysson Muotri
contact affiliation	Director Sanford Stem Cell Education and Integrated Space Stem Cell Orbital Research (ISSCOR) Center Director Gene Therapy Initiative
contact email	muotri@health.ucsd.edu
lab head
Paulo Carvalho
contact affiliation	Fiocruz
contact email	paulo@pcarvalho.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD069807

PRIDE project URI

• PRIDE
  1. PXD069807
     1. Label: PRIDE project
     2. Name: Proteomic dataset of MECP2-deficient and wild-type human brain organoids under spaceflight and ground conditions