PXD069804

PXD069804 is an original dataset announced via ProteomeXchange.

Title	Neutrophil-Derived Apoptotic Bodies Function as Decoy Vesicles to Preserve Bone Integrity during Infection
Description	Bone infection represents a pathological intersection of immunity and skeletal remodeling, where inflammatory responses drive excessive bone loss. Neutrophils dominate the early immune infiltration during infection, yet how their fate within infected bone marrow influences disease progression remains poorly understood. Here, we identify neutrophil-derived apoptotic bodies (Neut-ABs) as critical mediators that preserve bone integrity during infectious osteomyelitis. Bone infection triggers extensive neutrophil apoptosis, resulting in the accumulation of Neut-ABs. Integrated transcriptomic and proteomic profiling of these Neut-ABs revealed significant enrichment of TLR2 and sialyltransferase ST3GAL1, and lectin blotting confirmed that TLR2 is modified with α2,3-linked sialic acids. Mechanistically, α2,3-sialylated TLR2 on Neut-ABs functions as a molecular decoy that competitively interferes with Siglec15–TLR2 interactions in osteoclast precursors, thereby preventing precursor fusion and suppressing bone-resorptive activity. This inhibitory effect requires both the formation and sialylation of Neut-ABs, as it was abolished in Fas-deficient (MRL/Lpr) mice, upon neuraminidase-mediated desialylation, or when using Neut-ABs derived from Tlr2⁻/⁻ mice. Therapeutic delivery of Neut-ABs markedly attenuates osteolysis in infected mice without altering bacterial clearance. These findings uncover a neutrophil-to-bone apoptotic signaling axis in which sialylated vesicular decoys selectively suppress osteoclast activation, illuminating a new paradigm linking innate immune turnover to skeletal homeostasis.
HostingRepository	iProX
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_18:36:35.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qixiu Yu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 18:35:32	ID requested
⏵ 1	2025-10-22 18:36:35	announced

Dataset with its publication pending

submitter keyword: Neutrophils, Apoptotic bodies, Osteomyelitis, Sialylation, Osteoclastogenesis, Bone homeostasis

Ce Dou
contact affiliation	First Affiliated Hospital of Army Medical University
contact email	lance.douce@gmail.com
lab head
Qixiu Yu
contact affiliation	First Affiliated Hospital of Army Medical University
contact email	763365254@qq.com
dataset submitter

iProX dataset URI