PXD069776

PXD069776 is an original dataset announced via ProteomeXchange.

Title	Integrative Proteomics and Lipidomics Analysis of Post-Myocardial Infarction Patients Treated With PCSK9 Antibodies and Statins
Description	Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibition is a potent cholesterol-lowering strategy. This study examined effects of statins and PCSK9 monoclonal antibodies (mAbs) beyond low-density lipoprotein cholesterol reduction, which are not fully defined. To complement in vivo plasma proteomic data, we used HepG2 human hepatoma cells stably expressing apolipoprotein(a) (LPA). Cells were exposed to escalating doses of atorvastatin for ten days, followed by collection of conditioned media and cell lysates for quantitative mass spectrometric analysis. Proteomic profiling of cell lysates revealed a dose-dependent effect on HMG-CoA reductase and other enzymes within the mevalonate pathway. Analysis of conditioned media demonstrated a strong, dose-dependent induction of procollagen C-endopeptidase enhancer 1 (PCOLCE) and a modest increase in secreted LPA. These findings corroborate clinical observations that statin therapy can modestly elevate circulating lipoprotein(a) [Lp(a)] and is associated with increased plasma PCOLCE levels. The HepG2 data provide evidence for a predominant hepatic origin of this PCOLCE response, representing a novel mechanistic insight into statin-associated proteomic changes.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_17:08:36.771.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD069776
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	XIAOKE YIN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 07:05:41	ID requested
⏵ 1	2026-04-26 17:11:38	announced

10.1161/circgen.125.005345;

10.6019/PXD069776;

Schmidt LE, Burnap SA, Singh B, Takov K, Losdat S, Schrutka L, Galli L, Theofilatos K, Otto GW, Hengstenberg C, Tzoulaki I, Lang IM, Koskinas KC, Speidl WS, R, ä, ber L, Mayr M, Integrative Proteomic and Lipidomic Analysis of Patients With Acute Myocardial Infarction Treated With PCSK9 Antibodies and Statins. Circ Genom Precis Med, 19(2):e005345(2026) [pubmed]

submitter keyword: Human, LPA, hepatoma cells, LCMS, DDA, HepG2

Prof Manuel Mayr MD PhD
contact affiliation	National Heart and Lung Institute, Imperial College London, United Kingdom
contact email	m.mayr@imperial.ac.uk
lab head
XIAOKE YIN
contact affiliation	National Heart and Lung Institute, Faculty of Medicine, Imperial College London
contact email	xiaoke.yin@imperial.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069776
     1. Label: PRIDE project
     2. Name: Integrative Proteomics and Lipidomics Analysis of Post-Myocardial Infarction Patients Treated With PCSK9 Antibodies and Statins