PXD069774

PXD069774 is an original dataset announced via ProteomeXchange.

Title	Localized Wnt-signaling promotes asymmetric NuMA-dependent oriented divisions and unequal apportioning of mitochondria
Description	In multicellular organisms, the execution of developmental and homeostatic programs often rely on asymmetric cell divisions. These divisions require the alignment of the mitotic spindle axis to cortical polarity cues, and the unequal partitioning of cellular components between progeny cells. Asymmetric divisions are orchestrated by niche signals frequently presented in a directional manner, such as Wnt signals. Here we employ bioengineered Wnt-niches to demonstrate that in metaphase NuMA/dynein microtubule motors form a complex with Lrp6 and -catenin at the cortical sites of Wnt activation to orient cell division perpendicularly. We show that engagement of Lrp6 receptors by Wnt ligands locally stabilizes actomyosin contractility through the accumulation of myosin-1c. Additionally, we developed a proteomic-based approach to identify mitotic protein networks enriched at the Wnt-contact site, revealing that mitochondria polarize toward localized Wnt sources and are asymmetrically partitioned to the Wnt-proximal daughter cell during Wnt-mediated asymmetric cell division of embryonic stem cells. Our findings enhance the understanding of mitotic Wnt-signaling, and elucidate fundamental principles underlying Wnt-dependent cell polarization.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_16:31:42.052.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069774
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	simone tamburri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-22 06:39:31	ID requested
⏵ 1	2025-12-07 16:31:43	announced

Eli S, Rauso G, Ghezzi P, Szczerkowski JLA, Bruzzi M, Rizzelli F, Iommazzo F, Loffreda A, Castagna F, Don, à F, Gaddoni C, Dondi A, Marenda M, Rodighiero S, Tournier P, Lavagnino Z, Parazzoli D, Gauthier NC, Tamburri S, Pasini D, Habib SJ, Mapelli M, Localized Wnt-signaling promotes asymmetric NuMA-dependent oriented divisions and unequal apportioning of mitochondria. Nat Commun, 16(1):10690(2025) [pubmed]

10.6019/PXD069774;

10.1038/s41467-025-65775-z;

submitter keyword: Human, Wnt, NuMA, MassSpectrometry

Marina Mapelli
contact affiliation	IEO, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, Italy.
contact email	marina.mapelli@ieo.it
lab head
simone tamburri
contact affiliation	European Institute of Oncology (IEO)
contact email	simone.tamburri@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD069774

PRIDE project URI

• PRIDE
  1. PXD069774
     1. Label: PRIDE project
     2. Name: Localized Wnt-signaling promotes asymmetric NuMA-dependent oriented divisions and unequal apportioning of mitochondria