PXD069744

PXD069744 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of GAP-binding proteins in colorectal cancer reveals PPP1CA as a direct target of microbial GABA-mediated metabolic rewiring
Description	This dataset corresponds to the proteomic analysis presented in Figure 6A of the manuscript “Obesity-driven microbial GABA depletion promotes metabolic rewiring and colorectal cancer progression.” We performed Drug Affinity Responsive Target Stability (DARTS) and co-immunoprecipitation proteomics using colorectal cancer cell lysates incubated with glyceraldehyde-3-phosphate (GAP) to identify GAP-binding proteins. Mass spectrometry data revealed PPP1CA as a key GAP-interacting protein mediating GABA-dependent suppression of YAP signaling. The dataset supports the mechanistic link between microbiota-derived GABA, TPI1-generated GAP, and YAP-regulated tumor metabolism in obesity-associated colorectal cancer.
HostingRepository	PRIDE
AnnounceDate	2026-06-21
AnnouncementXML	Submission_2026-06-21_04:33:38.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dawei Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 13:50:46	ID requested
⏵ 1	2026-06-21 04:33:39	announced

Dataset with its publication pending

submitter keyword: GABA

GAP

PPP1CA

Jingxin Li
contact affiliation	Shandong University
contact email	ljingxin@sdu.edu.cn
lab head
Dawei Chen
contact affiliation	IKMB, Kiel University
contact email	greenlandice@live.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD069744

PRIDE project URI

• PRIDE
  1. PXD069744
     1. Label: PRIDE project
     2. Name: Proteomic identification of GAP-binding proteins in colorectal cancer reveals PPP1CA as a direct target of microbial GABA-mediated metabolic rewiring