PXD069740

PXD069740 is an original dataset announced via ProteomeXchange.

Title	Structural variation at lhcb6 underlies genetic variation in photosystem II maximum quantum efficiency in maize
Description	Targeted utilization of native genetic diversity can expand the genetic basis of traits that exhibit limited genetic variation in elite breeding material and can enhance our understanding of the genotype-phenotype relationships associated with complex traits in crops. In a genome-wide association study in a European maize landrace we identified quantitative trait loci (QTL) that affected the maximum quantum efficiency of photosystem II (Fv/Fm) in field experiments. In a forward genetic approach, we focused on a QTL on chromosome 10, explaining a large proportion of the genetic variance for Fv/Fm in growth stages V4 (35%) and V6 (47%), for genetic dissection and candidate gene discovery. Integrating molecular and physiological information we show that allelic variation at the gene encoding LIGHT HARVESTING CHLOROPHYLL A/B BINDING PROTEIN6 (LHCB6 also known as CP24), a component of the photosystem II (PSII) light-harvesting complex (LHCII) antenna, underlies the variation in Fv/Fm. We demonstrate that the allelic variation results from a hAT transposon insertion at lhcb6 and is associated with differential accumulation of the LHCII antenna components LHCB6 and LHCB3, leading to impaired non-photochemical quenching (NPQ) and reduced plant biomass accumulation. Based on proteomic analyses we propose candidate genes that compensate the unfavorable growth effects caused by impaired LHCII antenna assembly. Our work provides novel insights into the function of lhcb6 in the context of LHCII antenna assembly and demonstrates the value of natural variation in landraces for the understanding and genetic improvement of complex photosynthetic processes.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_04:50:38.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lukas Würstl
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: NEWT:4577;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 13:09:27	ID requested
⏵ 1	2026-03-16 04:50:40	announced

10.1038/s41598-026-42348-8;

Urzinger S, W, ü, rstl L, Avramova V, Urbany C, Scheuermann D, Presterl T, Reuscher S, Mayer M, Brajkovic S, K, ü, ster B, Ouzunova M, Ordas B, Westhoff P, Sch, ö, n CC, Native alleles at lhcb6 shape photosynthetic efficiency and early growth in maize. Sci Rep, 16(1):(2026) [pubmed]

submitter keyword: photosynthesis,Maize

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany
contact email	Kuster@tum.de
lab head
Lukas Würstl
contact affiliation	Chair of plant breeding Technical University of Munich (TUM)
contact email	l.wuerstl@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD069740

PRIDE project URI

• PRIDE
  1. PXD069740
     1. Label: PRIDE project
     2. Name: Structural variation at lhcb6 underlies genetic variation in photosystem II maximum quantum efficiency in maize