PXD069724

PXD069724 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Downstream Proteins Regulated by THRAP3 in THP-1 Cells
Description	This study aimed to analyze the downstream proteins regulated by THRAP3 in THP-1 cells using proteomics. THP-1 cells were divided into control and KO groups (with 3 biological replicates each, transfected with sgNC or sgTHRAP3 respectively). After cell lysis, trypsin digestion, Tandem Mass Tags labeling, fractionation, and resuspension, peptide fractions were separated via LC and analyzed by MS on a Q-Exactive HF Orbitrap mass spectrometer. MS data were interpreted with Percolator v.355 to identify UniProt sequences with a 1% FDR.
HostingRepository	PRIDE
AnnounceDate	2025-12-11
AnnouncementXML	Submission_2025-12-11_00:50:21.021.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pan Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 05:54:00	ID requested
⏵ 1	2025-12-11 00:50:22	announced

10.1038/s41467-025-66931-1;

Wang D, Wu Z, Liu S, He J, Zhang H, Chen P, Yang M, THRAP3 promotes ferroptosis resistance in acute myelocytic leukemia through SLU7-mediated alternative splicing of GIT2. Nat Commun, 17(1):235(2025) [pubmed]

submitter keyword: proteomics,THRAP3, Tandem Mass Tags, THP-1 cells, LC-MS/MS

Pan Chen
contact affiliation	Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 138, Tongzipo Road, Changsha, Hunan 410013, China
contact email	chenpan08@csu.edu.cn
lab head
Pan Chen
contact affiliation	Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University
contact email	chenpan08@csu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD069724

PRIDE project URI

• PRIDE
  1. PXD069724
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Downstream Proteins Regulated by THRAP3 in THP-1 Cells