PXD069715

PXD069715 is an original dataset announced via ProteomeXchange.

Title	Multi-Omics Reveals that Alkaline Mineral Complex Enhances Nutrient Digestion–Absorption and Gut Health
Description	3.This study, conducted under a high-concentrate finishing context, used an integrated multi-omics approach to evaluate the effects of an Alkaline Mineral Complex (AMC) on jejunal digestion–absorption and gut health in beef cattle. The results showed that AMC alleviated mucosal pathological injury and inflammatory cell infiltration in the jejunum, significantly reduced crypt depth and increased the villus height/crypt depth (VH/CD) ratio (p = 0.0004 and 0.0002, respectively), and modulated redox homeostasis, evidenced by a significant decrease in MDA accompanied by concurrent reductions in SOD and CAT, with T-AOC and GSH-Px remaining overall unchanged (MDA, SOD, CAT all p < 0.0162; others p > 0.05). Pro-inflammatory cytokines IL-6 and IL-1β showed downward trends, whereas TNF-α slightly increased; none reached statistical significance (p > 0.05). While overall α-diversity and community structure did not separate between groups, the relative abundances of Olsenella and Cyanobacteria were higher in the AMC group (p < 0.05), and enrichment of Massilia/Oxalobacteraceae/Burkholderiales in controls suggested stronger Proteobacteria-related signals. Metabolomics achieved OPLS-DA separation and identified 28 differential metabolites, enriching pathways such as “Protein digestion and absorption.” Proteomics identified 382 differentially expressed proteins, enriched in carbon metabolism, glycolysis/gluconeogenesis, pentose phosphate pathway, fatty-acid degradation, glutathione metabolism, as well as tight junction and AMPK/insulin signaling. Functionally, digestive enzyme activities showed overall upward but non-significant trends, whereas nutrient-sensing/transport and barrier-related molecules (GPR43, FABP2, SNAT2, LAT2, ZO-1) were significantly upregulated (all p < 0.05). Integrating multi-omics and phenotypic evidence, AMC appears to enhance jejunal digestion–absorption and improve gut health by metabolic remodeling and barrier strengthening, jointly optimizing the “oxidative stress–microbiome/metabolite–nutrient transport” axis.
HostingRepository	iProX
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_01:32:14.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Gu
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 01:31:59	ID requested
⏵ 1	2025-10-21 01:32:15	announced

Dataset with its publication pending

submitter keyword: 4.Intestinal Health，Nutrient Transporters，High-Concentrate Diet

Zhicai Zuo
contact affiliation	Sichuan Agricultural University
contact email	czyhzzc@126.com
lab head
Li Gu
contact affiliation	Sichuan Agricultural University
contact email	1030448279@qq.com
dataset submitter

iProX dataset URI