PXD069711

PXD069711 is an original dataset announced via ProteomeXchange.

Title	RNF26 Bridges ER Stress and Autophagy in the Clearance of MERS Envelope protein
Description	Coronavirus envelope (E) proteins are small, highly conserved viroporins essential for virion assembly and pathogenicity. Despite extensive characterization of their ion channel activity, how host cells sense and dispose of excessive viral membrane proteins remains poorly understood. Here we show that expression of the MERS-CoV E protein triggers pronounced ER stress and autophagy activation in human cells. The E protein is selectively degraded through an RNF26-dependent autophagy–lysosome pathway, and inhibition of autophagy or loss of RNF26 function leads to E accumulation and sustained unfolded protein response. Mechanistically, RNF26, an ER-anchored E3 ubiquitin ligase, mediates ubiquitin tagging of the viral protein and spatially coordinates autophagic flux to facilitate its clearance. Disruption of this process establishes a self-amplifying ER stress–autophagy feedback loop that exacerbates proteotoxicity. These findings define a membrane homeostatic conflict between viral viroporins and the host defense machinery, and identify RNF26 as a potential therapeutic target for mitigating viroporin-induced cytotoxicity through host-directed intervention.
HostingRepository	iProX
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_01:20:34.060.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziyue Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-21 01:20:17	ID requested
⏵ 1	2025-10-21 01:20:34	announced

Dataset with its publication pending

submitter keyword: Coronavirus envelope (E) proteins, Autophagy, Host-virus interaction, Cellular stress response, Pathogenicity

Jingbo Qie
contact affiliation	Fudan University
contact email	jingboqie@fudan.edu.cn
lab head
Ziyue Li
contact affiliation	Fudan University
contact email	liziyue177@163.com
dataset submitter

iProX dataset URI