PXD069676 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic and ubiquitinomics of HUH7 cells after treatment of A1 |
| Description | Protein ubiquitination is a crucial post-translational modification that regulates diversecellular processes, including protein degradation and signal transduction. This study employs proteomic and ubiquitinomic analyses to systematically profile the alterations in protein expression and ubiquitination sites in Huh7 cells after treatment with 10 µM DUBTAC A1.We utilized high-resolution tandem mass spectrometry (LC-MS/MS) followed by extensive bioinformatic analysis. The dataset comprises raw mass spectrometry data, processed search results including protein identification and quantification, and a comprehensive list of identified ubiquitination sites. Our data provides a valuable resource for understanding the crosstalk between proteomic and ubiquitinomic alterations. This repository contains the complete proteomic and ubiquitinomic dataset supporting the findings of the related manuscript. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-24 |
| AnnouncementXML | Submission_2025-11-23_16:19:17.939.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chen He |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | ubiquitinylated lysine |
| Instrument | Orbitrap Astral; LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-20 09:54:49 | ID requested | |
| ⏵ 1 | 2025-11-23 16:19:19 | announced | |
Publication List
| 10.1002/anie.202518249; |
| He C, Li J, Cui C, Xie J, Feng J, Zhang B, Huang X, Ma R, Zheng L, Xu J, Yao H, Xu S, Construction of Small-Molecule Deubiquitinase-Targeting Chimeras to Reactivate and Stabilize Mutant p53 Y220C in Vitro and in Vivo. Angew Chem Int Ed Engl, 65(3):e18249(2026) [pubmed] |
Keyword List
| submitter keyword: A1, ubiquitinomics, Proteomic,Huh7 |
Contact List
| He Chen |
|---|
| contact affiliation | School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, Jiangsu 211198, China |
| contact email | hechen285@126.com |
| lab head | |
| Chen He |
|---|
| contact affiliation | China Pharmaceutical University |
| contact email | hechen285@126.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD069676 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069676
- Label: PRIDE project
- Name: Proteomic and ubiquitinomics of HUH7 cells after treatment of A1
