PXD069675

PXD069675 is an original dataset announced via ProteomeXchange.

Title	Chronic colitis induces a diverse neuroinflammatory fingerprint and myeloid cell-mediated dopaminergic neuron loss in the midbrain
Description	Chronic gut inflammation is associated with neuroinflammation and brain diseases. Yet, the regional pattern and single-cell resolution of the brain immune response to chronic gut inflammation remains elusive. Combining confocal microscopy and multiomic analyses, we observed that chronic colitis-derived systemic inflammation results in a midbrain-specific inflammatory response characterized by an inflammatory shift of microglial clusters, CD8+ T cell extravasation, and increased numbers of vessel-associated neutrophil granulocytes. Myeloid cell targeting after colitis onset resulted in a 67% reduction of midbrain microglia and prevented dopaminergic neuron loss in the substantia nigra pars compacta. Collectively, we provide the first single-cell resolution of an innate and adaptive immune response to chronic gut inflammation in the midbrain and identified a detrimental role of myeloid cells in midbrain dopaminergic neuron loss. These results substantially advance the understanding of the gut-immune-brain axis that links peripheral inflammation to distinct neuroinflammatory and neurodegenerative processes related to Parkinson’s disease.
HostingRepository	PRIDE
AnnounceDate	2026-07-13
AnnouncementXML	Submission_2026-07-13_04:38:27.545.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Kielkowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-20 09:47:04	ID requested
⏵ 1	2026-07-13 04:38:28	announced

10.1186/S12974-026-03926-9;

submitter keyword: neuroinflammation

neurodegeneration

midbrain

microglia

T cells

neutrophils

gut-immune-brain axis

inflammatory bowel disease

Pavel Kielkowski
contact affiliation	LMU Munich, Department of Chemsitry
contact email	pavel.kielkowski@cup.lmu.de
lab head
Pavel Kielkowski
contact affiliation	LMU Munich
contact email	pavel.kielkowski@cup.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD069675

PRIDE project URI

• PRIDE
  1. PXD069675
     1. Label: PRIDE project
     2. Name: Chronic colitis induces a diverse neuroinflammatory fingerprint and myeloid cell-mediated dopaminergic neuron loss in the midbrain