PXD069669

PXD069669 is an original dataset announced via ProteomeXchange.

Title	Proteomics of The multinutrient Fortasyn Connect influences gut microbiota and intestinal function in early Alzheimer´s disease
Description	Nutritional supplementation is emerging as a promising strategy to support the clinical management of early Alzheimer’s disease (AD), partly through modulation of the intestinal microbiome via the microbiota–gut–brain axis. This study investigated the impact of Fortasyn Connect (Souvenaid®), a multinutrient formulation, on the gut microbiota using a dual approach: i) a dynamic gastrointestinal simulator (simgi®) inoculated with fecal samples from AD patients, and ii) an observational study involving early-stage AD patients (n = 22) receiving or not the supplement. The in vitro model provided a host-independent assessment of microbiota responses, revealing increased Bifidobacterium and Lactobacillus levels, along with enhanced short-chain fatty acid (SCFA) production. In patients, supplementation was associated with higher fecal abundance of Bifidobacterium and Christensenellaceae, reduced inflammatory markers (calprotectin and myeloperoxidase), and elevated butyrate levels. Fecal lipidomic and proteomic analyses indicated improved lipid digestion, increased secretory IgA, and modulation of host proteins related to gut–brain homeostasis. Systemically, higher circulating levels of iron, folate, and vitamin B12 were also observed. This study demonstrates that multinutrient supplementation such as Fortasyn Connect can beneficially modulate the gut ecosystem and immune–metabolic pathways in early AD, targeting disease-relevant mechanisms through the gut–brain axis in the context of aging.
HostingRepository	PRIDE
AnnounceDate	2025-12-03
AnnouncementXML	Submission_2025-12-03_07:27:36.946.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069669
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lucía Méndez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-20 08:22:15	ID requested
⏵ 1	2025-12-03 07:27:37	announced

10.6019/PXD069669;

10.14336/AD.2025.1176;

submitter keyword: Human, Alzheimer’s disease, proteomics, nutritional supplementation, feces, gut microbiota, label-free quantification, Fortasyn Connect, spectral counting, LC–MS/MS

Lucía Méndez
contact affiliation	Institute of Marine Research (IIM-CSIC), Spain
contact email	luciamendez@iim.csic.es
lab head
Lucía Méndez
contact affiliation	Institute of Marine Research (IIM-CSIC)
contact email	luciamendez@iim.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069669
     1. Label: PRIDE project
     2. Name: Proteomics of The multinutrient Fortasyn Connect influences gut microbiota and intestinal function in early Alzheimer´s disease