PXD069663

PXD069663 is an original dataset announced via ProteomeXchange.

Title	Proteomics of human iPSC derived dopaminergic neurons with a Parkinson's disease linked PARK7/DJ-1 mutation
Description	Parkinson’s disease (PD) is characterized by α-synuclein accumulation and dopaminergic neuron degeneration, with dopamine (DA) oxidation emerging as a key pathological driver. However, the mechanisms underlying this neurotoxic process remain unclear. Using PD patient-derived and CRISPR-engineered iPSC midbrain dopaminergic neurons lacking DJ-1, we identified defective sequestration of cytosolic DA into synaptic vesicles, which culminated in DA oxidation and α-synuclein accumulation. In-depth proteomics, state-of-the-art imaging, and ultrasensitive DA probes uncovered that decreased VMAT2 protein and function impaired vesicular DA uptake, resulting in reduced vesicle availability and abnormal vesicle morphology.
HostingRepository	PRIDE
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-07_04:43:04.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-20 03:38:56	ID requested
⏵ 1	2026-01-07 04:43:05	announced

Dataset with its publication pending

submitter keyword: VMAT2, DJ-1,Parkinson’s Disease, dopaminergic neurons, PARK7, iPSC derived neurons

Stefan F. Lichtenthaler
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen-Str. 17, 81377 München, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD069663

PRIDE project URI

• PRIDE
  1. PXD069663
     1. Label: PRIDE project
     2. Name: Proteomics of human iPSC derived dopaminergic neurons with a Parkinson's disease linked PARK7/DJ-1 mutation