PXD069635

PXD069635 is an original dataset announced via ProteomeXchange.

Title	Proteomics studies of the The GT1 domain of RNase J in Arabidopsis
Description	This study investigates the function of the C-terminal GT1 domain of chloroplast RNase J in Arabidopsis thaliana, focusing on its role in RNA quality control and double-stranded RNA (dsRNA) metabolism. To characterize the molecular effects of GT1 domain disruption, comparative proteomic analyses were performed on transgenic Arabidopsis seedlings expressing FLAG-tagged RNase J variants: wild type (WTc), GT1 domain deletion (ΔGT), and GT1 tryptophan-mutated (mGT) forms that are deficient in nucleic acid binding.The resulting proteomic dataset supports the conclusion that loss or mutation of the GT1 domain does not markedly alter chloroplast protein accumulation, despite significant effects on chloroplast dsRNA levels and transcriptome organization. These findings establish a link between RNase J’s GT1 domain, dsRNA recognition, and plastid RNA quality control.
HostingRepository	PRIDE
AnnounceDate	2026-01-18
AnnouncementXML	Submission_2026-01-18_00:12:01.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-19 15:31:00	ID requested
⏵ 1	2026-01-18 00:12:01	announced

10.1093/NAR/GKAG033;

submitter keyword: Chloroplast,RNase J, RNA quality control, GT1 domain

Gadi Schuster
contact affiliation	Faculty of Biology, Technion-Israel Institute of Technology, Haifa 3200003, Israel
contact email	gadis@technion.ac.il
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069635
     1. Label: PRIDE project
     2. Name: Proteomics studies of the The GT1 domain of RNase J in Arabidopsis