PXD069620

PXD069620 is an original dataset announced via ProteomeXchange.

Title	Structure and organisation of AMPA receptor-TARP complexes in the mammalian cerebellum
Description	AMPA receptors (AMPARs) are multimodal transducers of glutamatergic signals throughout the brain. Their diversity is exemplified in the cerebellum, the brain structure integrating sensorimotor information. At afferent synapses, AMPARs mediate high-frequency excitation, while in Bergmann glia (BG) they support slow calcium transients that modulate synaptic transmission. This spectrum arises from different combinations of core subunits (GluA1-4), auxiliary proteins, and post-transcriptional modifications. Here, using mass-spectrometry, cryo-EM, and electrophysiology, we characterize major cerebellar AMPARs: low calcium-permeable GluA2/A4 heteromers with four TARP subunits, mainly neuronal in origin, and BG-specific calcium-permeable GluA1/A4 heteromers containing only two Type-2 TARPs. Moreover, GluA4 receptors consistently exhibit compact N-terminal domains that promote synaptic delivery. Our study defines the organisational principles of native AMPAR complexes in the mammalian cerebellum and reveals how different receptor subtypes support cell-type specific functions.
HostingRepository	PRIDE
AnnounceDate	2026-02-26
AnnouncementXML	Submission_2026-02-26_01:16:32.083.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069620
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sew Peak-Chew
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-18 14:30:35	ID requested
⏵ 1	2026-02-26 01:16:32	announced

10.6019/PXD069620;

10.1126/SCIENCE.AEB3577;

submitter keyword: AMPA glutamate receptor, mammalian cerebellum, cry-EM

Ingo Greger
contact affiliation	MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus CB2 0QH Cambridge
contact email	ig@mrc-lmb.cam.ac.uk
lab head
Sew Peak-Chew
contact affiliation	MRC-LMB
contact email	spc@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069620
     1. Label: PRIDE project
     2. Name: Structure and organisation of AMPA receptor-TARP complexes in the mammalian cerebellum