PXD069609 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics characterization of the inner membrane domain (IMD) from Mycobacterium tuberculosis |
| Description | The inner membrane domain (IMD) is a metabolically active and laterally discrete membrane domain initially discovered in Mycobacterium smegmatis that correlates both temporally and spatially with polar cell envelope elongation. While it has remained unclear whether a similar membrane domain exists in pathogenic species, this study specifically demonstrates that the IMD is a conserved membrane structure found in Mycobacterium tuberculosis. Following isolation of the membrane domains by density gradient fractionation, proteomic analysis revealed that the IMD is enriched in metabolic enzymes that are involved in the synthesis of conserved cell envelope components such as peptidoglycan, arabinogalactan, and phosphatidylinositol mannosides. Further, by demonstrating that the IMD is concentrated in the polar region of the rod-shaped cells, where active cell envelope biosynthesis takes place, proteomic analysis further revealed the enrichment of enzymes involved in synthesis of phthiocerol dimycocerosates and phenolic glycolipids. Overall, these proteomic data support that the functional compartmentalization of the membrane is an evolutionarily conserved feature found in both M. tuberculosis and M. smegmatis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-21 |
| AnnouncementXML | Submission_2025-11-21_09:10:16.402.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD069609 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Scott Shaffer |
| SpeciesList | scientific name: Mycobacterium tuberculosis TBR27; NCBI TaxID: NEWT:1295743; |
| ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-17 17:34:01 | ID requested | |
| ⏵ 1 | 2025-11-21 09:10:17 | announced | |
Publication List
Keyword List
| submitter keyword: proteomics,Mycobacterium tuberculosis, inner membrane domain, infectious disease, microbiology |
Contact List
| Yasu S. Morita |
|---|
| contact affiliation | Department of Microbiology University of Massachusetts Amherst USA |
| contact email | ymorita@umass.edu |
| lab head | |
| Scott Shaffer |
|---|
| contact affiliation | University of Massachusetts Medical School |
| contact email | scott.shaffer@umassmed.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069609
- Label: PRIDE project
- Name: Proteomics characterization of the inner membrane domain (IMD) from Mycobacterium tuberculosis
