PXD069542

PXD069542 is an original dataset announced via ProteomeXchange.

Title	Pervasive phenotypic effects of FBXO42 promoted by regulation of PP4 phosphatase
Description	F-box proteins are the substrate recognition modules of the SCF (SKP1–Cullin–F-box) E3 ubiquitin ligase complex. FBXO42, an understudied member of this family, has recently emerged as a modulator of key cellular processes, including cell cycle progression, DNA damage response and glioma stem cell survival. In this study, we define the function of FBXO42 as a major regulator of the protein phosphatase PP4. Phosphoprotein phosphatases (PPPs) have a broad array of substrates, hence necessitating tight regulation. We observe that FBXO42 ubiquitinates the PP4 complex to govern the assembly of regulatory and catalytic subunits with the net effect of restraining the latter’s phosphatase activity. FBXO42 depletion unleashes PP4 activity with broad cellular effects highlighting FBXO42 as a novel regulatory node in ubiquitin-mediated signalling for future therapeutic exploitation.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:35:53.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	iolanda Vendrell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-16 12:31:30	ID requested
⏵ 1	2026-02-22 16:35:54	announced

10.1038/s44318-025-00675-y;

Yang H, Smith P, Ma Y, Southworth E, Gopala Krishna V, Salerno B, Rowland J, Loftus AEP, Grieco D, Vendrell I, Fischer R, Kessler BM, D'Angiolella V, Pervasive phenotypic effects of FBXO42 are promoted by regulation of PP4 phosphatase. EMBO J, 45(4):1332-1361(2026) [pubmed]

submitter keyword: cancer, Fbxo42, SCF, PPP phosphatases,ubiquitin

Vincenzo D’Angiolella
contact affiliation	Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK
contact email	vdangio@ed.ac.uk
lab head
iolanda Vendrell
contact affiliation	Target Discovery Institute, NDMRB
contact email	iolanda.vendrell@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD069542

PRIDE project URI

• PRIDE
  1. PXD069542
     1. Label: PRIDE project
     2. Name: Pervasive phenotypic effects of FBXO42 promoted by regulation of PP4 phosphatase