PXD069526

PXD069526 is an original dataset announced via ProteomeXchange.

Title	The yeast peroxisomal proteome at absolute quantitative scale
Description	Peroxisomes are dynamic organelles vital for lipid metabolism and redox homeostasis. In Saccharomyces cerevisiae, the expression of peroxisomal proteins is tightly regulated in response to metabolic conditions. Here, we provide the first absolute quantification of the yeast peroxisomal proteome under peroxisome-inducing (oleate) and fermentative (glucose) conditions using a label-free mass spectrometry approach. We determined protein copy numbers for ~4,500 proteins, including 99 peroxisomal and peroxisome-associated proteins. Our data reveal that the peroxisomal proteome is ~3-fold higher abundant in oleate-grown cells, constituting 2.8% of the total proteome (corresponding to 2.01 x 10E6 protein copies) compared to 0.8% (6.67 x 10E5 protein copies) in glucose, reflecting the necessity for peroxisomal functions such as fatty acid beta-oxidation. Enzymes of the beta-oxidation and glyoxylate cycle showed up to >500-fold higher abundance in oleate. In contrast, core components of the peroxisomal protein import machinery (e.g., Pex5, Pex14) exhibited only moderate changes (~2- to 8-fold). In addition to metabolic enzymes and components of the peroxisomal protein import pathways, we provide copy number data for proteins involved in cellular stress response, peroxisome proliferation, division and organization, peroxisome-associated membrane contact sites, and metabolite transporter. Taken together, our dataset offers a quantitative framework of peroxisomal remodeling under different metabolic conditions and highlights the organelle’s adaptive flexibility, providing a valuable resource for future studies on peroxisome biology.
HostingRepository	PRIDE
AnnounceDate	2026-02-16
AnnouncementXML	Submission_2026-02-16_06:13:10.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Bender
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-16 06:37:38	ID requested
⏵ 1	2026-02-16 06:13:11	announced

Das H, Oeljeklaus S, Maier R, Bender J, Warscheid B, The yeast peroxisomal proteome at absolute quantitative scale. Histochem Cell Biol, 164(1):8(2026) [pubmed]

10.1007/s00418-026-02458-w;

submitter keyword: Saccharomyces cerevisiae

peroxisomes

protein copy numbers

absolute quantification

proteomic ruler

mass spectrometry

Bettina Warscheid
contact affiliation	Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany
contact email	bettina.warscheid@uni-wuerzburg.de
lab head
Julian Bender
contact affiliation	University of Wuerzburg Chair of Biochemistry II Am Hubland 97074 Würzburg
contact email	julian.bender@uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069526
     1. Label: PRIDE project
     2. Name: The yeast peroxisomal proteome at absolute quantitative scale