PXD069515

PXD069515 is an original dataset announced via ProteomeXchange.

Title	tRF-Glu-TTC exacerbates intestinal ischemia/reperfusion injury via ADAR1/autophagy/ROS/necroptosis signaling axis
Description	Using tRNA-derived stress-induced RNA (tiRNA) and tRNA-derived fragment (tRF) sequencing, we identified tRF-Glu-TTC as significantly upregulated in intestinal tissues, plasma, epithelial cells, and human clinical samples. tRF-Glu-TTC levels positively correlated with the severity of intestinal injury. Functionally, tRF-Glu-TTC exacerbates intestinal mucosal damage by activating TNF pathway-mediated necroptosis in intestinal epithelial cells. Mechanistically, tRF-Glu-TTC directly binds to and suppresses the RNA-editing enzyme ADAR1, impairing adenosine-to-inosine editing of autophagy-related transcripts (ATG18 and Lamp2), thereby suppressing autophagy and concurrently triggering reactive oxygen species (ROS) accumulation. This cascade promoted TNF-α secretion, amplifying TNF pathway-mediated necroptosis.
HostingRepository	iProX
AnnounceDate	2025-10-16
AnnouncementXML	Submission_2025-10-16_00:25:42.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenjuan Zhang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-16 00:25:29	ID requested
⏵ 1	2025-10-16 00:25:42	announced

Dataset with its publication pending

submitter keyword: tsRNA, tRF-Glu-TTC

ADAR1, necroptosis, intestinal I/R

Kexuan Liu
contact affiliation	Nanfang Hospital, Southern Medical University
contact email	liukexuan705@163.com
lab head
Wenjuan Zhang
contact affiliation	Nanfang Hospital, Southern Medical University
contact email	845897843@qq.com
dataset submitter

iProX dataset URI