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PXD069513
PXD069513 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Spatiotemporal Proteomic Atlas of Lipid Droplets in Haematococcus pluvialis During Encystment |
| Description | This project presents a comprehensive spatiotemporal proteomic analysis of lipid droplets (LDs) isolated from Haematococcus pluvialis across five distinct life cycle stages: green vegetative motile (HPLDs-1), green nonmotile (HPLDs-2), brown nonmotile (HPLDs-3), orange nonmotile (HPLDs-4), and red nonmotile cyst (HPLDs-5). Using TMT-based quantitative proteomics, we identified and quantified 3,396 high-confidence LD-associated proteins, revealing dynamic stage-specific proteome remodeling during the encystment process. Functional annotations and enrichment analyses highlight the roles of LDs in lipid metabolism, carotenoid biosynthesis, energy homeostasis, vesicular transport, and oxidative stress response. Notably, we observed significant enrichment of chloroplast-, mitochondrial-, and ER-derived proteins, as well as ribosomal components, suggesting LDs function as multifunctional metabolic hubs that actively coordinate astaxanthin accumulation and cellular adaptation to stress. This dataset provides a valuable resource for understanding LD biology in microalgae and supports further efforts in metabolic engineering for astaxanthin production. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-16 |
| AnnouncementXML | Submission_2026-07-01_01:08:35.543.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qunju Hu |
| SpeciesList | scientific name: Haematococcus; NCBI TaxID: 44744; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-16 00:23:36 | ID requested | |
| ⏵ 1 | 2026-07-01 01:08:36 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Haematococcus pluvialis, Lipid droplets, Proteomics, Encystment, Astaxanthin, Metabolic hubs, Spatiotemporal dynamics, Stress adaptation |
Contact List
| Xiaojun Yan | |
|---|---|
| contact affiliation | Zhejiang Ocean University |
| contact email | yanxj@zjou.edu.cn |
| lab head | |
| Qunju Hu | |
| contact affiliation | Zhejiang Ocean University |
| contact email | huqunju2022@zjou.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
