PXD069498

PXD069498 is an original dataset announced via ProteomeXchange.

Title	Extracellular Vesicles from the Probiotic Yeast Pichia kudriavzevii: Proteomic Characterization and Modulation of Immune and Defense Responses in an Induced Inflammation Model of Intestinal Epithelial Cells
Description	Extracellular vesicles (EVs) derived from probiotics represent a new and exciting frontier in host-microbe therapeutics. These nanoscale carriers are not merely cellular byproducts but are sophisticated mediators of intercellular communication, capable of modulating immune responses, reducing inflammation, and inhibiting pathogens through a rich cargo of bioactive molecules. The EVs isolated from the culture supernatants of the yeast probiotic candidate Pichia kudriavzevii were characterized for their dimensions, protein composition, and targeting both the gut pathogen virulence and the host inflammatory response. The vesicles had a size distribution from 100 to 150 nm, which is consistent with previous reports on fungal EVs. Proteomic analysis of the purified EVs identified a complex array of 189 proteins, hypothesized to be responsible for some of the antimicrobial and immunomodulatory properties observed. Safety was a key consideration, and the isolated EVs demonstrated no cytotoxicity in human CaCo-2 cells and no in vivo toxicity in the Galleria mellonella larval model, confirming their potential for safe use. The field is moving towards a new era of "postbiotics," where cell-free therapies offer a safer, more stable alternative to live probiotics.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:51:10.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CHIARA D'AMBROSIO
SpeciesList	scientific name: Pichia kudriavzevii; NCBI TaxID: NEWT:4909;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-15 16:48:44	ID requested
⏵ 1	2026-04-05 16:51:11	announced

10.3390/nu18060912;

Maione A, Matuozzo M, Imparato M, D'Ambrosio C, de Alteriis E, Guida M, Scaloni A, Galdiero E, : Proteomic Characterization and Modulation of Immune and Defense Responses in an Induced Inflammation Model of Intestinal Epithelial Cells. Nutrients, 18(6):(2026) [pubmed]

submitter keyword: probiotics

Pichia kudriavzevii

membrane vesicles

proteomics

immuno-inflammatory responses

Chiara D'Ambrosio
contact affiliation	Proteomic, Metabolomic and Mass Spectrometry LAB, ISPAAM, NATIONAL RESEARCH COUNCIL
contact email	chiara.dambrosio@cnr.it
lab head
CHIARA D'AMBROSIO
contact affiliation	ISPAAM CNR
contact email	chiara.dambrosio@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069498

PRIDE project URI

• PRIDE
  1. PXD069498
     1. Label: PRIDE project
     2. Name: Extracellular Vesicles from the Probiotic Yeast Pichia kudriavzevii: Proteomic Characterization and Modulation of Immune and Defense Responses in an Induced Inflammation Model of Intestinal Epithelial Cells