⮝ Full datasets listing
PXD069476
PXD069476 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Asymmetric biphasic electric stimulation supports cardiac maturation and functionality |
| Description | Two-dimensional (2D) cardiac models are widely used for cardiotoxicity screening but often lack structural and functional maturity of adult native tissue. Electrical stimulation (ES) enhances in vitro maturation, yet conventional waveforms (monophasic and symmetric biphasic) have shown limitations, including charge accumulation and possible cell hyperpolarization. Here, we introduce for the first time an asymmetric biphasic ES waveform that combines the advantages of monophasic and symmetric biphasic stimulation by reversing the current and reducing residual voltage. Asymmetric biphasic stimulation improved electrical functionality, calcium handling and contractility of neonatal rat cardiac cells, without triggering cellular stress. Additionally, cells subjected to asymmetric biphasic ES displayed a metabolic shift toward fatty acid oxidation, a hallmark of mature cardiomyocytes. Taken together, these findings highlight the novelty and efficacy of asymmetric biphasic stimulation in generating more physiologically relevant in vitro cardiac models, providing a promising alternative to standard ES protocols. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-01 |
| AnnouncementXML | Submission_2025-12-01_01:38:27.492.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | danilo ritz |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-15 01:37:39 | ID requested | |
| ⏵ 1 | 2025-12-01 01:38:27 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: electrical stimulation, monophasic waveform, biphasic waveform, asymmetric biphasic waveform, cardiac tissue engineering, DatasetType:Proteomics |
Contact List
| Alexander Schmidt | |
|---|---|
| contact affiliation | Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland |
| contact email | alex.schmidt@unibas.ch |
| lab head | |
| danilo ritz | |
| contact affiliation | University of Basel |
| contact email | danilo.ritz@unibas.ch |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099494/ |
